Nitrogen can not only promote the vegetative growth of wine grape，but also effectively regulate its reproductive growth. Nitrogen supplement period and nitrogen supplement methods are particularly important for coordinating the relationship between nutritional physiology and quality composition of grape，especially in the most critical color turning period of grape quality formation. Nitrogen regulation has the most significant effect on the composition of berries quality. Taking 8-year-old wine grape cultivar ‘ Cabernet Sauvignon ’ as the research object and water as the control，four nitrogen supplement methods including foliar spraying，drip irrigation，spray-drop combination and reduced nitrogen supplement were set up to study the effects of nitrogen supplement methods on grape physiology，quality and yeast assimilation nitrogen. The results showed that compared with spraying water，the four nitrogen supplement methods improved the fruit shape index of grapes. Drip irrigation nitrogen supplement effectively improved the leaf area，water use efficiency and chlorophyll b content，water use efficiency significantly increased by 81.53%. The tannins，anthocyanins and total phenols of grape berries significantly increased by 11.69%，99.19% and 21.60% with foliar spraying nitrogen. Reducing nitrogen supplement significantly promoted the synthesis and accumulation of chlorophyll，while soluble solids significantly increased by 16.15%. The combination of spraying and dripping had the best effect on improving net photosynthetic rate，carotenoid and assimilatable nitrogen content in leaves，which increased by 35.15%，38.46% and 53.04%，respectively，and 100-grain weight increased by 44.88%. Comprehensive evaluation showed that nitrogen supplement through foliar spraying and drip irrigation was an effective way to regulate grape physiology and improve grape berry quality in the eastern foot of Helan mountain.