In order to improve the ability of plant growth promoting rhizobacteria (PGPR) strains to produce ACC deaminase，three PGPR strains were optimized for optimal enzyme production process and maize proliferation experiments.Based on the single-factor experiment，the effects of pH，temperature，ACC substrate concentration，NaCl concentration and shaker speed on the enzyme activity of ACC deaminase were investigated，and the optimal enzyme production process was optimized in response surface with pH，temperature and ACC substrate concentration as the main factors.The final enzyme production conditions were pH 8，temperature 32 ℃，ACC deaminase substrate concentration 6.0 mmol/L for W5；pH 10，temperature 30 ℃，ACC deaminase substrate concentration 6.0 mmol/L for strain BS2-2；pH 9，temperature 30 ℃，ACC deaminase substrate concentration 6.0 mmol/L for strain 5#.The enzymatic activity was 6.6，2 and 4-fold higher than that before optimization.In the pot experiment，the W5 strain was the most effective in promoting growth.The use of PGPR with high ACC deaminase production can promote maize growth，which can lay the foundation for making as microbial compound.