外源菌群及与植物联合修复华北中度石油污染土壤效果研究
作者:
作者单位:

(1.清华大学天津高端装备研究院应用生物技术与装备研究所,天津 300300;2.中国农业科学院农业资源与农业区划研究所,北京 100081)

作者简介:

孙婧(1986-),副研究员,博士,主要从事环境及农业微生物研究。E-mail:sunjing@tsinghua-tj.org。

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基金项目:

基金项目:河北省重点研发计划项目资源与环境专项(20373804D)。


Effects of exogenous petroleum degrading microbial consortium and phyto-microbial remediation on medium level petroleum contaminated soil from North China
Author:
Affiliation:

(1.Research Center for Applied Biological Technology and Equipment,Tianjin Research Institute for Advanced Equipment,Tsinghua University,Tianjin 300300;2.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081)

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    摘要:

    为探讨不同微生物菌群对华北地区中度石油污染土壤的修复效果,在实验室模拟条件下分别进行优势外源石油降解菌群的筛选和优势菌群与植物联合修复试验。结果显示:4种外源石油降解菌群中,PDC-3菌群对中度石油污染土壤总石油烃(TPH,total petroleum hydrocarbons)去除率为84.07%,在各菌群中效果最优;该优势菌群与植物联合修复中度石油污染土壤比单独使用优势菌群修复可获得更好的效果,优势菌群与黑麦草联用及优势菌群与紫花苜蓿联用120 d TPH的去除率分别为91.58%和89.30%,修复后土壤中TPH含量均小于500 mg/kg;同时优势菌群与黑麦草联合修复在90 d即可去除89.32%的土壤TPH,相比选用紫花苜蓿可有效缩短修复周期;优势菌群对土壤TPH的去除起主要作用,其贡献率远高于土著微生物菌群或植物的贡献率;植物对土壤TPH去除的贡献率为4.09%~6.48%,且其作用主要发生在修复过程的中后期;优势菌群单独使用或与植物联合修复中度石油污染土壤120 d均可有效去除C10~C12及C22~C40石油烃组分,去除率为85.14%~100.00%;然而,C13~C21石油烃组分含量表现出阶段性的积累效应;除对土壤石油烃的去除作用外,使用优势菌群进行生物修复存在一定的调节土壤pH、增加土壤肥力,且有利于恢复修复后的土壤功能。

    Abstract:

    The remediation effects of petroleum degrading microbial consortium on medium level petroleum contaminated soil from North China was studied through exogenous petroleum degrading microbial consortiums screening and laboratory simulation of phyto-microbial remediation. The results indicated:TPH degrading efficiency of PDC-3 microbial consortium was 84.07%,which was the highest among the four microbial consortiums screened. In the laboratory remediation simulation,after 120 d,PDC-3 phyto-microbial treatments achieved better remediation effects than that of PDC-3 treatment alone. The TPH degrading efficiencies of PDC-3 with Lolium perenne L. planting and PDC-3 with Medicago sativa L.planting were 91.58% and 89.30% respectively,and the soil TPH were both reduced to lower than 500 mg/kg. Moreover,PDC-3 with L. perenne planting reached up to 89.32% TPH degrading efficiency in 90 d,and required shorter remediation period than that of PDC-3 with M. sativa planting. Furthermore,PDC-3 was the major factor in soil TPH degrading process,its TPH degrading contribution rate was much higher than that of endogenous microbial consortiums or plants;Plants contributed up to 4.09%~6.48% of the soil TPH degraded,and it functioned mostly in the late stage of the remediation process. Meanwhile,PDC-3 microbial consortium effectively removed 85.14%~100.00% C10~C12 and C22~C40 petroleum hydrocarbons in 120 d when used alone as well as in phyto-microbial remediation. However,the C13~C21 petroleum hydrocarbons showed a temporary accumulation effect. At last,soil pH and fertility were improved during phyto-microbial remediation using PDC-3,which would be important for soil function restoration.

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孙婧,蔡典雄,蒋广勇,任会战,吴会军.外源菌群及与植物联合修复华北中度石油污染土壤效果研究[J].中国土壤与肥料,2023,(3):210-217.

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  • 收稿日期:2022-03-03
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  • 录用日期:2022-03-17
  • 在线发布日期: 2023-06-27
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