The paper aims to study the effects of biogas slurry application on the contents and forms of soil nitrogen in cropland，and to provide data support for sustainable utilization of biogas slurry farmland.The data were obtained from the published papers by searching the key words including “Biogas slurry” and “Soil nitrogen” in “CNKI” and “Web of Science” database before 2021.With Meta-analysis，quantitative effects of biogas liquid application on soil nitrogen contents，microbial community and crop yield were investigated.Compared with chemical fertilizer treatments，biogas slurry application significantly reduced nitrate nitrogen contents and increased the contents of alkaline nitrogen and microbial biomass nitrogen in soil，and improved communities of bacteria，fungal and actinomycetes in soil and crop yield.Compared with the mixed slurry of cattle，swine and chicken manure，swine manure biogas slurry significantly reduced nitrate nitrogen contents.Low input of biogas slurry nitrogen with ≤50 kg·hm-2 significantly reduced the contents of nitrate nitrogen and ammonium nitrogen，while increased total nitrogen contents in soil.The ratio of biogas slurry nitrogen replacing fertilizer nitrogen with 20%～40% increased the contents of total nitrogen and alkaline nitrogen in soil and the number of microbial communities，while reduced the nitrate nitrogen.In all，biogas slurry application significantly affected soil nitrogen content，but did not increase the risk of nitrogen leaching.In the application of biogas slurry，the application of swine manure biogas slurry，controlling the proportion of biogas slurry nitrogen instead of chemical fertilizer nitrogen to 20%～40% and ≤50 kg·hm-2 biogas slurry nitrogen input will also significantly reduce the risk of nitrogen leaching.