In order to explore the effects of different reducing fertilizer application modes on yield，nutrient use efficiency in rice-wheat cropping system to provide theoretical basis for achieving efficient application of chemical fertilizer and its zero increase in China，this paper carried out a 3-year field positioning experiment of rice-wheat cropping system. The experiment set 6 treatments，including no fertilizer(T1)，conventional chemical fertilization(T2)，combined application of organic manure and chemical fertilizer(T3)，reducing 20% chemical nitrogen fertilizer plus increasing nitrogen fertilizer synergist application(T4)，reducing 20% chemical phosphorus fertilizer along with phosphorus activator application(T5)，and reducing 20% chemical nitrogen fertilizer and 20% chemical phosphorus fertilizer along with nitrogen fertilizer synergist plus phosphorus activator application(T6). The results showed that compared with T2，the effect of fertilizer reduction on rice-wheat yield was not significant. T4 treatment improved the partial productivity and the agronomic use efficiency of nitrogen and potassium，the absorption efficiency of nitrogen and phosphorus，and the total uptake of phosphorus. T5 treatment significantly increased the total phosphorus uptake，phosphorus use efficiency，partial productivity of phosphorus，agronomic use efficiency of phosphorus，and agronomic use efficiency and the partial productivity of potassium in crops. T6 treatment significantly improved the nitrogen，phosphorus and potassium use efficiency and agronomic use efficiency of crops，and improved the partial productivity of nitrogen and potassium. T3 treatment had no significant effect on the uptake and utilization of nitrogen and phosphorus，but significantly increased the partial productivity of potassium in wheat and rice. In conclusion，the T6 treatment was beneficial to coordinate nutrient uptake and stabilize wheat and rice yield. It could be a preference pattern for reducing fertilizer application and increasing efficiency in rice-wheat cropping system at the middle and lower reaches of the Yangtze River and other regions with similar ecological conditions.