In order to clarify the effects of biodegradable amino acid fertilizer from feather on pakchoi yield and quality，pot experiments of equal nitrogen treatment with amino acid fertilizer(T2)and urea(T3)were conducted. The water treatment (T1)was set as control. The effects of different fertilizer application on growth and photosynthesis parameters，as well as quality of pakchoi were studied. The results showed that nitrogen supply promoted the shoot growth of pakchoi significantly，comparing with T1 treatment，the shoot dry mass of T2 and T3 treatments increased by 17% and 38%，respectively. Significant positive relationships were observed between leaf number/ leaf area and shoot dry mass. Both shoot nitrogen accumulation and net photosynthesis rate enhanced with nitrogen addition，and there was no significant difference in net photosynthesis rate between T2 and T3 treatments. A positive relationship was existed between shoot nitrogen accumulation and shoot dry mass. The contents of carotenoid，soluble protein，flavonoid and phenolic were not significantly affected by different treatments. Comparing with T3 treatment，amino acids content increased while NO^3- content decreased significantly in T2 treatment. Therefore，both the yield and quality of pakchoi increased with the supply of biodegradable amino acid fertilizer from feather，which has a good prospect of applying in vegetable production as a commercial fertilizer.