To optimize soybean nutrients management measures，field experiments were conducted in Zhoukou of Henan province and Harbin of Heilongjiang province to study the application effect of Nutrient Expert system combined with microbial agents on reducing fertilization rates and improving efficiency.Five treatments were set up as follows:Nutrient Expert system recommended fertilization(NE)，70% NE(70% of N rate of NE treatment，nitrogen reduction，NR)，70% NE + PGPR(RP)，70% NE + Rhizobium(RR)and 70% NE + PGPR + Rhizobium(RPR).Results showed that NR treatment reduced soybean yield by 14.4%～23.3%，and decreased N uptake by 15.0%～16.8% and phosphorus(P)uptakes by 16.7%～26.9% than NE treatment，respectively.The RPR attained the highest yield across all treatments，which was 40.9% higher than NE at Zhoukou and was similar to that of NE at Harbin.Compared with NR treatment，the yield of RP and RR treatments were increased by 7.2% and 32.4% at Zhoukou，while thosew were increased 12.9% and 6.2% at Harbin，respectively.Different treatments had no significant effects on soil NH4+-N；however，RR treatment decreased soil NO^3--N by 24.1%～34.4% than that of NE treatment，and RPR treatment increased available P by 89.8% at Zhoukou and increased available potassium(K)by 37.5% at Harbin，respectively，than that of NE treatment.In conclusion，the Nutrient Expert system combined with microbial agents increased soil available P and K，promoted nutrients uptake and high yield of soybean，and which can further reduce fertilization rates while achieving high soybean yield.