A laboratory experiment was conducted to study the effect of carbendazim on soil nitrogen cycling and biological properties under different soil moisture conditions，which was of great significance for evaluation of the effects of carbendazim on soil nitrogen cycle and ecological environment.This study quantified the effects of successive carbendazim applications on soil nitrogen mineralization，chitinase activity，soil urease kinetic parameters and microbial biomass under different soil moisture.The successive carbendazim applications generated significant effects on soil ammonium nitrogen，nitrate nitrogen，chitinase activity and microbial biomass nitrogen，and the interaction of soil moisture and carbendazim addition also significantly affected on ammonium nitrogen，nitrate nitrogen，mineralization rate，urease initial rate of enzyme catalysis (Vmax/Km) and microbial biomass nitrogen.The successive carbendazim applications at the rate of 5 mg·kg-1 dry soil significantly increased the ammonium contents under the condition of 60% maximum water-holding capacity(WHC)，and the successive carbendazim applications at the rate of 25 mg·kg-1 dry soil significantly increased the nitrate contents under different soil moisture conditions.Under the condition of 60% WHC，the mineralization rate of the treatment with 10 mg·kg-1 dry soil carbendazim was significantly higher than those of the without carbendazion treatments，but the successive carbendazim applications at the rates of 10 and 25 mg·kg-1 dry soil significantly decreased the mineralization rate at 90% WHC.Soil chitinase activity decreased with the increase of carbendazim dosage under different soil moisture conditions.The value of urease Vmax/Km in the treatment with 25 mg·kg-1 dry soil carbendazim was significantly lower than that in the treatment with 10 mg·kg-1 dry soil carbendazim under the condition of 60% WHC.The successive carbendazim applications could affected soil urease kinetic parameters by affecting soil mineral nitrogen contents and biological properties.