In order to explore the effect of microbial fertilizer on soil fertility，yield and quality of tea in tea garden，the tea cultivar ‘Longjing 43’ was used as material.The effects of no fertilizer(CK)，conventional fertilizer(TF)，multi-strain compound microbial fertilizer(BCF1)，soybean meal fermentation microbial fertilizer(BCF2)and fulvic acid microbial fertilizer(BCF3)on soil nutrient content，carbon，nitrogen and phosphorus invertase activity and green tea yield and quality in tea garden were studied by field experiment.The results showed that compared with conventional fertilizers，three microbial fertilizers increased soil pH by 0.27 units on average，decreased bulk density by 9.73%，and increased soil available potassium，available phosphorus，organic matter and alkali-hydrolyzable nitrogen by 9.72%，6.25%，9.19% and 5.40% on average，respectively.Compared with conventional fertilizers，three microbial fertilizers increased the activities of β-glucosidase and β-N-acetylglucosaminidase by 11.61% and 20.13%，respectively，and fulvic acid microbial fertilizers increased the activities of leucine aminopeptidase，cellulose diglucosidase and β-xylanase by 97.70%，66.44% and 71.62%，respectively.Compared with conventional chemical fertilizer，three microbial fertilizer treatments increased the phosphorus accumulation of tea by 10.08%，and increased the contents of iron，calcium，manganese and nickel by 19.63%，7.56%，12.06% and 24.14%，respectively.Compared with conventional fertilizers，fulvic acid microbial fertilizers significantly increased hundred-bud weight，germination density，fresh leaf yield and total free amino acids.Correlation analysis showed that fresh leaf yield was significantly positively correlated with soil available potassium，available nitrogen，organic matter，cellulose diglucosidase，acid phosphatase and β-xylanase enzyme activities(P<0.05).In summary，compared with chemical fertilizers，the three microbial fertilizers can better promote the soil fertility of tea gardens and improve the yield and quality of tea.Among them，fulvic acid microbial fertilizers have the best effect.