In order to explore the effect of the application of sulfur fertilizer in the agricultural production on the growth of spring maize and the utilization efficiency of nitrogen fertilizer under different levels of nitrogen，phosphorus and potassium fertilizer，six treatments were set up，including low nitrogen，phosphorus and potassium level + no sulfur application(LS0)，low nitrogen，phosphorus and potassium level + sulfur fertilizer(LS6)，medium nitrogen，phosphorus and potassium level + no sulfur application(MS0)，medium nitrogen，phosphorus and potassium level + sulfur fertilizer(MS6)，high nitrogen，phosphorus and potassium level + no sulfur application(HS0)，high nitrogen，phosphorus and potassium level + sulfur fertilizer(HS6)，to study their effects on soil alkaline nitrogen，maize nitrogen accumulation，grain yield and nitrogen fertilizer utilization efficiency. The results showed that increasing the fertilization level of nitrogen，phosphorus and potassium significantly increased the alkaline nitrogen content of soil. Applying sulfur fertilizer at the same level of nitrogen，phosphorus and potassium fertilization had no effect on soil alkaline nitrogen content. There was a significant positive correlation between the total nitrogen accumulation of aboveground or grain yield of spring maize and the fertilization level of nitrogen，phosphorus and potassium and the application of sulfur fertilizer. Compared with only nitrogen，phosphorus and potassium treatments，the total nitrogen accumulation of nitrogen，phosphorus and potassium combined with sulfur fertilizer increased significantly by an average of 10.24%，and the grain yield increased significantly by an average of 5.70%. However，with the increase of nitrogen，phosphorus and potassium fertilization levels，the effect of sulfur fertilizer on nitrogen accumulation and grain yield gradually decreased. The productivity，agronomic efficiency and utilization rate of nitrogen fertilizer with the same sulfur application level gradually decreased with the increase of nitrogen，phosphorus and potassium fertilization level. At the same level of nitrogen，phosphorus and potassium，sulfur application improved the nitrogen fertilizer productivity，agricultural efficiency and utilization rate of spring maize. The above results showed that with the increase of nitrogen，phosphorus and potassium fertilization，the alkaline nitrogen content of soil，the accumulation of aboveground nitrogen and grain yield of spring maize gradually increased，but the utilization efficiency of nitrogen fertilizer of spring maize gradually decreased. The application of sulfur fertilizer can further increase the absorption and accumulation of nitrogen and grain yield on the ground of spring maize on the basis of increasing the level of nitrogen，phosphorus and potassium fertilizer，and at the same time improve the utilization efficiency of nitrogen fertilizer to a certain extent.