To investigate the effects of combined application of organic fertilizer and nitrogen(N)，phosphorus(P)，potassium(K)fertilizers on maca quality and yield，and to provide reference for high quality Lepidium meyenii planting and rational fertilization，six treatments were set up in the field experiment:CK，M(organic fertilizer)，F1(conventional fertilization)，F1+M，F2(50% conventional fertilization)and F2+M. Fertilization significantly increased the agronomic traits，yield and quality of Lepidium meyenii shoots and roots. In August and September，shoot growth was promoted，while in October，Lepidium meyenii root growth was accelerated. The demand for P and K was higher in the early growth stage of Lepidium meyenii，but the demand for N was higher in the whole growth stage. Fertilization significantly increased the contents of macamides，macaenes，glucosinolate compounds and ash of Lepidium meyenii，F1+M treatment showed the highest contents of macamides，macaenes and ash，which were 192.79，7.93 and 3.97 mg/100 g，while F1 treatment showed the highest contents of glucosinolate compounds，which was 2.47 mg/100 g. Among all fertilization treatments，F1+M treatment had the highest yield，which was 11708 kg/hm². The results showed that the yield and quality of Lepidium meyenii could reach the best under F1+M treatment，that is，chemical fertilizer N 120，P2O5 90，K2O 120 kg/hm²，combined with 7500 kg of refined organic fertilizer.