Exploring Trichoderma like biological fungal fertilizer in soil improvement has become a research focus. In order to study the improvement effect of different Trichoderma spp. on soil of ginseng cultivation，the soil physicochemical properties，and soil fungal community structure and function were analyzed. Ginseng pot experiment was conducted to analyze soil physical and chemical properties and soil microbial community diversity in which exogenous Trichoderma strains (four strains of T. hamatum DH3，MM3，MM4，Q7 and one strain of T. harzianum MM6) were added. The results showed that the soil physicochemical properties of Panax ginseng were changed by different exogenous Trichoderma strains. The soil pH did not change significantly，and the total nitrogen，organic matter，ammonium nitrogen，nitrate nitrogen，available phosphorus and available potassium were increased significantly(P< 0.05)，while soil carbon nitrogen(C/N) ratio was decreased in the treatments with addition of Trichoderma strains. Generally speaking，the soil physicochemical properties treated by T. hamatum MM3 and T. harzianum MM6 were the best. Using Illumina NovaSeq high-throughput sequencing platform，the sequence of ITS variation region of soil fungi was determined，and 703 OTUs were obtained from all samples.Fungal species detected in these samples covered 8 phyla，26 classes，46 orders，86 families and 141 genera.The soil fungi α diversity index (ACE，Chao1) increased，especially in the treatment of T. hamatum MM3. The dominant fungal community were Hypocreaceae and Trichoderma in the treatment of T. hamatum MM3，and were Chaetomiaceae in T. harzianum MM6. Redundancy analysis (RDA) ascertained that soil C/N ratio and nitrate nitrogen were the main factors affecting the soil microbial diversity. The study provided theoretical basis for soil microbial improvement and biological control of cultivated ginseng.