The effects of different carriers on the stability of Bacillus subtilis GY801，the ability of soil multiplication，the ability of plant growth promotion，the ability of bacteriostasis and disease resistance were investigated. The results showed that the addition of sodium lactate to the amino acid trace elements (AA-TE) liquid fertilizer significantly increased the 6-month retention rate of bacillus subtilis GY801 to 87.07%. The addition of sodium lactate to the AA-TE liquid fertilizer significantly increased the retention rate of bacillus subtilis GY801 to 87.07%，compared with 46.39% of the control group，the retention rate of the bacteria increased by 87.7%(P<0.01)，and the effects of different carriers on the multiplication ability of the bacteria were compared under the suitable conditions of soil multiplication，the results showed that the multiplication of GY801 in soil could be significantly increased by adding seaweed concentrate. The multiplication of GY801 in soil was 110，57 and 51 times in the control group at 7，15 and 30 days，respectively，and increased to 154，94 and 79 times with the addition of seaweed concentrate，respectively. The growth-promoting effect of GY801 was significantly enhanced by seaweed carrier compared with the control group without carrier，and the growth-promoting effect of GY801 was increased by 40.0%，64.9% and 54.9%，respectively. The indexes of healthy seedling index，root-shoot ratio，dry weight of whole plant and dry weight of root were increased by 7.7%，8.3%，10.1% and 16.7%，respectively (P<0.05). Compared with the control group，the control effect of soybean meal on root rot was 66.8% after 30 days，which was 4.06 times higher than that of the control group. The results showed that sodium lactate was the most suitable carrier for liquid bacterial agent，seaweed extract was the most suitable carrier for soil proliferation and growth promotion，and soybean meal was a relatively suitable carrier for bacteriostasis and disease prevention.