The content of soil available phosphorus (AP) is one of the important factors to evaluate soil fertility and fertilization guidance. The normal operation (Olsen-P determined by artificial molybdenum-antimony colorimetric method combined with sodium bicarbonate extraction) is complicated，and takes more time. Improving the efficiency and accuracy of the conventional testing methods is an urgent technical problem to be solved in the soil testing and formula fertilization. This study was conducted to determine the AP contents in four groups of soil samples with different AP content using SmartChem 200 method. The results showed that the AP had good linear correlations in the range of 0.00~2.00 mg/L，and the correlation coefficient was 1. There were no significant difference between determination results of the SmartChem 200 method and those of the national standard method，and the relative errors were between 0.00%~2.07%. The AP content of national standard substance (NSA-2) results showed that the relative standard deviation was 0.37%，and the adding standard recoveries were between 99.00%~101.00%. The determination of soil AP by automatic chemical analyzer had high accuracy and precision，and the results could meet the requirements for soil AP testing. With the simple operation and high testing efficiency，the SmartChem 200 method can handle large numbers of soil samples.