In order to solve the problem of excessive application of chemical fertilizer in grape production in Hexi Corridor，five different base fertilization treatments were set up，including chemical fertilizer，corn straw，farm manure，biological organic fertilizer of Parch company and biological organic fertilizer of Angel yeast company. The effects of different treatments on grape fruit quality were studied after continuous application for three years. The results showed that compared with application of chemical fertilizer，the application of organic fertilizer improved the quality of grapes in varying degrees，and the effect of biological organic fertilizer was better than straw and farm manure；Among the two kinds of biological organic fertilizers，biological organic fertilizer of Parch company had the best effect. The fruit size，functional substance and soluble sugar content of biological organic fertilizer of Parch company were significantly higher than those of application of chemical fertilizer. Among them，the single fruit weight，vertical and horizontal diameter of the fruit increased by 34.53%，24.89% and 20.39%，respectively，the total sugar content of the fruit increased by 37.29%，and the organic acid content decreased by 52.99%. To sum up，in the facility production of 'Sunshine Rose' grape，increasing the application of organic fertilizer has a good effect on improving the fruit quality，and the selection of organic fertilizer should be biological organic fertilizer containing mineral source potassium fulvic acid.