The objectives of this study were to understand the effects of selenium application on the selenium accumulation and transformation from inorganic to organic form in Anoectochilus roxburghii and the influence of selenium on its growth. Sodium selenite application test was conducted by adding to seedling propagation medium，transplanting substrate，humus soil under the forest and by foliage spraying，and then the relationship between the indexes of the shape and main components of A. roxburghii，total selenium and organic selenium of plant and organs，growth amount of plant and selenium application amount were detected and analyzed. The results showed that selenium application significantly increased the selenium content of the plants and the highest absorption and utilization rate of selenium was 13.09% for adding to seedling propagation medium，followed by foliage spraying，adding to transplanting substrate and humus soil under the forest，which were 6.81%，6.36% and 2.79%，successively. The total selenium content of dry plant increased with the application concentration，the organic selenium content was 81.8%-92.1%，and the selenium organic conversion rate was not affected by the application concentration. The selenium content in each organ of the plant ranked as root>leaf>stem，and the conversion rate of organic selenium in plant organs ranked as leaf>root>stem. The proper concentration of selenium applied to the root could promote the growth of A. roxburghii，while excessive concentration could inhibit its growth. Foliage spray selenium had no significant effect on the growth of the plants. Selenium application had no significant effect on the content of main components in the plants. The best economic application amount of selenium in the plants of A. roxburghii was 4.75 mg/L in seedling propagation medium，0.168 mg/plant in foliage spraying，8.00 mg/L in transplanting substrate and 9.50 mg/L in humus soil under the forest. From the comprehensive application effect，the best absorption and utilization efficiency and the best yield effect could be obtained by adding selenium to the seedling propagation medium.