设施尾菜高温闷棚还田对土壤微生物群落结构的影响
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(1.湖北省农业科学院植保土肥研究所,湖北 武汉 430064;2.农业农村部废弃物肥料化利用重点实验室,湖北 武汉 430064;3.武汉市农业科学院,湖北 武汉 430345)

作者简介:

徐大兵(1983-),副研究员,博士,主要从事农业废弃物资源化利用研究。E-mail:dabing_xu@163.com。

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基金项目:湖北省农业科学院植保土肥研究所青年基金项目(2021ZTSQJ06);湖北省现代农业产业技术体系(HBHZD-ZB-2020-007)。


Effects of high temperature closed shed with vegetable returning on greenhouse soil microbial diversity and community structure
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(1.Institute of Plant Protection andSoil Fertilizer,Hubei Academy of AgriculturalSciences,Wuhan Hubei430064;2.Key Laboratory of Fertilization fromAgriculturalWastes,Ministry of Agriculture and RuralAffairs,Wuhan Hubei430064;3.Wuhan Academy of AgriculturalSciences,Wuhan Hubei430345)

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    摘要:

    为了揭示设施尾菜高温闷棚还田对土壤微生物群落结构的影响,采用田间试验,研究了直接高温闷棚、尾菜还田后高温闷棚以及尾菜还田配施腐熟菌剂后高温闷棚处理对土壤化学性质、细菌和真菌群落结构及其代谢功能的影响。结果表明:直接高温闷棚显著降低活性有机碳含量,减少细菌多样性而增加真菌多样性。尾菜高温闷棚还田以及配施腐熟菌剂增加了土壤碱解氮和速效钾含量,但是均降低了真菌多样性指数。高温闷棚前后细菌和真菌群落结构均发生了显著的变化。闷棚后主要降低了Bacteroidota、Acidobacteriota 和Crenarchaeota 丰度,但是真菌Ascomycota 丰度增加了16.42 ~ 34.01 个百分点,且尾菜还田的增加幅度更大。闷棚后镰刀菌属丰度降低了1.46 ~ 7.03 个百分点,而不闷棚则增加了0.46 个百分点。与直接闷棚相比,尾菜高温闷棚还田或者添加腐熟菌剂降低了反硝化功能、氨化功能和硝态氮还原功能基因的相对丰度,而只有尾菜高温闷棚还田降低了碳氢化合物、芳香化合物和纤维素降解功能基因的相对丰度,配施腐熟菌剂则又增加了这3 种相关功能基因的相对丰度。碱解氮是影响高温闷棚后土壤细菌和真菌群落结构的主要因子之一。由此可见,与直接高温闷棚相比,尾菜高温闷棚还田或者配施菌剂进一步改变了土壤微生物群落结构和相关碳氮代谢功能。

    Abstract:

    In order to reveal the effect of high-temperature closed shed with vegetable returning on greenhouse soil microbial communitystructure,the field experiments were carried out to study the effects of high-temperaturetreatments(direct high-temperature closedshed,high-temperature closed shed with vegetable returning and high-temperature closed shedafter vegetable returning with compostingagents,marking asT1,T2 andT3,respectively)on greenhouse soil chemicalproperties,bacterial and fungal community structure and metabolicfunction.The results showed that the direct high temperature closed shed significantly reduced the contentof active organic carbon and bacterialdiversity,but increased thefungal diversity.The soil alkali-hydrolyzable nitrogen and available potassium were increased by T2andT3,but both the fungal diversity index werereduced.The community structure of bacteria and fungi changed significantly before and after high temperature closedshed.The abundance ofBacteroidota,Acidobacteriota and Crenarchaeota were decreased afterclosedshed,but the abundance of Ascomycota wasincreased by16.42%-34.01%,and the greatest was obtained by hightemperature closed shed with vegetablereturning.The abundance of Fusarium was decreased by 1.46-7.03 percentage pointsafter closedshed,but increased by 0.46 percentage points without closedshed.Compared with the direct closedshed,the relative abundance of denitrificationfunction,ammonification function and nitrate nitrogen function genes reduced by the high temperature closed shed with vegetable returning orthe addition of compostingagents,while only the high temperature closed shed with vegetable returning reduced the relative abundance ofhydrocarbon,aromatic compounds and cellulose degradation functiongenes,and the application of composting agents increased the relative abundance of these three related carbon metabolism functionalgenes.Alkali -hydrolyzable nitrogen was one of the main factors affecting the community structure of soil bacteria and fungi after high temperature closedshed.Inconclusion,compared with the direct hightemperature closedshed,the soil microbial community structure and related carbon and nitrogen metabolism functions were changed by high temperature closed shed with vegetable returning orthe application of compostingagents.

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徐大兵,赵书军,佀国涵,袁家富,葛米红.设施尾菜高温闷棚还田对土壤微生物群落结构的影响[J].中国土壤与肥料,2023,(9):87-95.

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  • 收稿日期:2022-08-25
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  • 录用日期:2022-10-30
  • 在线发布日期: 2023-12-20
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