The influence of continuous cropping obstacles on maize seed production fields in Hexi Corridor is increasing year by year. How to effectively alleviate the continuous cropping obstacles and improve the quality and productivity of cultivated land was an urgent problem to be solved. In this study，a randomized block design was adopted in a maize planting field with continuous cropping for 18 years. Based on 100% conventional fertilizer (N 375.0 kg/hm²，P2O5 150.0 kg/hm²，K2O 120.0 kg/hm²) + organic fertilizer 15000.0 kg/hm²，four treatments were set up: CK(70% fertilizer + organic fertilizer)，T1 (70%chemical fertilizer + organic fertilizer + soil conditioner)，T2(70% chemical fertilizer + organic fertilizer + bio-fertilizer) and T3(70% chemical fertilizer + organic fertilizer + soil conditioner + bio-fertilizer)，and their effects on the physicochemical properties，microbial population changes，soil enzyme activity，and yield of continuous cropping soil in maize seed fields were studied. The results showed that different treatments had different ecological restoration effects on long-term continuous cropping soil of maize. Compared with CK， T1，T2 and T3 treatments had no obvious effect on soil alkaline hydrolysis nitrogen，available phosphorus and available potassium. Compared with CK，the soil bulk density and pH of T2 and T3 treatments were decreased significantly，soil total porosity， aggregate and saturated water capacity were increased significantly，soil organic matter，organic carbon and carbon supply were increased significantly，soil cation exchange capacity，sucrase，urease，phosphatase and catalase activity were increased significantly，fungi was decreased significantly，and bacteria and actinomyces were increased significantly. Compared with CK，T1，T2 and T3 treatments had certain effects on stem diameter，grain number and 1000-seed weight of maize. The stem diameter of T2 and T3 treatments were increased by 13.28% and 17.84%，the grain number were increased by 13.32% and 17.22%，1000-seed weight were increased by 3.53% and 4.07%，and the yield were increased significantly by 9.91% and 11.45%，respectively. Under the conditions of this experiment，T3 treatment had the best effects on ecological restoration and yield increase in maize seed continuous cropping soil.