In order to explore the pattern of nitrogen，phosphorus，potassium and dry matter absorption and accumulation of Pueraria thomsonii Benth. in lateritic red soil area，the “3414” randomized block design was used to study the nutrient absorption and accumulation characteristics of various organs in the key growth stages of Pueraria thomsonii Benth. under different fertilization levels. The results showed that the accumulation of nitrogen，phosphorus and potassium in Pueraria thomsonii Benth. increased the most from root formation to expansion period，and the accumulation curve was similar to “S-shaped”，and its absorption capacity was potassium > nitrogen > phosphorus. Under different nitrogen application levels，the nitrogen regulation effect of root tuber nitrogen content in seedling stage and root tuber expansion stage was more obvious， although the stem nitrogen content was significantly higher than the control. However，there was no significant difference between nitrogen application treatments. The nitrogen content of leaf at each growth stage was higher than that of root and stem. The nitrogen accumulation of reduced nitrogen application (N1) at maturity was the largest，which was 19.91 g/plant. Under different phosphorus application levels，the phosphorus content of root tuber at root formation and expansion stage， stem phosphorus content at root tuber formation stage，and leaf phosphorus content at maturity stage were significantly higher than those of the control. However，the regulation effect of phosphorus fertilizer in other growth stages was not obvious. The accumulation of phosphorus in the treatment of normal phosphorus application (P2) reached the peak of 2.04 g/plant at the root tuber expansion stage. Under different potassium application levels，after root tuber formation，the potassium content of each organ of K2 and K1 treatments increased more than that of K3 and K0 treatments，and K3 treatment reduced the potassium content of root tubers at maturity. The potassium accumulation showed as K2> K1> K0> K3> CK treatment，and there was a great difference in potassium accumulation of each treatment at maturity. From the seedling stage to the mature stage，the amount of dry matter of root tubers continued to rise，while the amount of dry matter of aboveground parts showed the accumulation characteristics of firstly increased and then decreased.The proportion of dry matter of root tubers gradually increased with the growth period. At maturity，root tubers accounted for 68.54%-78.56%，and aboveground parts accounted for 21.44%-31.46%. Among the different fertilization levels，the dry matter distribution of root tubers treated with reduced nitrogen application (N1P2K2) was the highest，accounting for 80.12%.