Soil active organic carbon is a relatively active chemical component in soil，which can reflect the availability of soil organic carbon(SOC)and can be used as an indicator of soil quality and fertility. The effects of grazing disturbance on soil active organic carbon in Leymus chinensis meadow steppe were explored，so as to understand the effect mechanism of grazing on carbon cycling in grassland ecosystem，and improve theoretical support for the ecological restoration process of degraded grassland. Based on the long-term beef cattle grazing platform of the National Field Scientific Observation and Research Station of Hulunbuir Grassland Ecosystem，six grazing intensities no grazing(G0.00)，slightly grazing(G0.23)，light grazing(G0.34)，moderate grazing(G0.46)，heavy grazing(G0.69)and extremely heavy grazing(G0.92)were set up，to analyze the changes of soil active organic carbon in Leymus chinensis meadow steppe under different grazing intensities and explore its correlation. The results showed that:(1)The contents of soil organic carbon and active carbon were significantly different among different soil layers，and the contents of soil organic carbon and active organic carbon [Particulate organic carbon(POC)，dissolued organic carbon(DOC)，microbial biomass carbon(MBC)and easily oxidizable organic carbon(EOC)] were decreased significantly with the increase of soil depth.(2)The proportion of active organic carbon in different soil layers was linearly positively correlated with grazing intensity，and the proportion of active organic carbon in soil was increased with the increase of grazing intensity. The proportion of active organic carbon in different soil layers under different grazing intensity was POC >EOC >MBC >DOC，and the proportion of EOC，MBC and POC were decreased with the increase of soil depth.(3)With the increase of grazing intensity，the above-ground biomass，underground biomass and soil moisture showed a downward trend.(4)Grazing intensity was significant negatively correlated with SOC and DOC. There was a significant positive correlation between above-ground biomass and soil carbon components. There was a very significant positive correlation between underground biomass and DOC. Soil temperature was negatively correlated with POC. Soil moisture and POC showed a very significant positive correlation，and was positively correlated with SOC. This study shows that grazing can reduce above-ground biomass，underground biomass and soil moisture. Moderate grazing can not only increase soil organic carbon and active carbon components，but also increase the proportion of soil active carbon，which conforms to the moderate disturbance hypothesis.