An experiment was conducted to understand the nitrogen absorption，utilization，transportation and yield status of regenerated rice under different cultivation methods，so as to provide scientific and theoretical basis for selecting reasonable soil cultivation methods for high yield cultivation of rice in southern regenerated rice areas. According to whether ploughing before winter and rotating tillage before transplanting，4 tillage methods were set up:ploughing once and rotary tilling once(T1)，ploughing once and rotary tilling twice(T2)，not ploughing and rotary tilling twice(T3)，and not ploughing and rotary tillage three times(T4)，the difference of dry matter accumulation in different organs of regenerated rice at different growth stages of rice plants under different tillage ways was observed，and the effects of different tillage methods on nitrogen uptake，transport，utilization efficiency and yield of regenerated rice were explored. Along with the advancement of the regenerated rice growth process，the accumulation of dry matter，nitrogen uptake at each growth stage showed a trend of increasing first and then decreasing，the performance of treatments showed as T2>T1>T4>T3. In terms of production effect，the combination of ploughing and rotary tillage was better than single rotary tillage. At the same number of tillage，increasing rotary tillage could promote crop growth，increase crop yield and partial productivity of nitrogen fertilizer. From the perspective of the impact of farming methods on the yields of early rice and regenerated rice，compared with T3 and T4，the yield of early rice of T2 was more significant，but there was no significant difference in the yield of regenerated rice. The impact of farming methods on the yield of early rice and ratoon rice was mainly in the first season，and the key was that T2 significantly increased the number of grains per panicle. From the perspective of nitrogen harvest index，nitrogen dry matter production efficiency and nitrogen grain production efficiency，the four farming methods showed a trend of T2>T1>T4>T3，with T2 being the best. Considering soil nutrient efficiency，nitrogen accumulation and transfer of rice，yield and its constituent factors，it is recommended to adopt ploughing once and rotary twice in the production of early rice and regeneration rice.