By investigating the effects of partial substitution of chemical fertilizer with organic fertilizer at different proportions on the quality of Jianggang tangerine and soil chemical properties，the suitable organic substitution ratio could be proposed to provide a theoretical basis for chemical fertilizer reduction and quality and yield enhancement technology for Jinggang tangerine. The field experiment was conducted in Xingan county in 2021. Five treatments were set up，including single application of chemical fertilizer(CK)，single application of organic fertilizer(T1)，85%(T2)，70%(T3)and 55%(T4)of chemical fertilizer nitrogen substituted by organic fertilizer. The results showed that the organic fertilizer substitution treatment had the positive effect on increasing the quality of Jinggang tangerine and soil nutrient content. Compare with CK，the contents of soil organic matter，alkali-hydrolyzable nitrogen，available potassium and available phosphorus of T3 treatment were increased by 9.64%，14.32%，12.45% and 19.46%，respectively. The organic fertilizer substitution for chemical fertilizer treatments effectively decreased the contents of available copper，cadmium，lead，mercury and arsenic in the soil，among which the T1 treatment had the lowest contents of the available heavy metals in soil. Soil enzyme activities showed different dynamic responses to different organic fertilizer substitution ratios，and the T3 treatment significantly enhanced the soil cellulase and nitrate reductase activities by 65.17% and 27.38%，compared with the CK treatment. The T3 treatment had a more significant effect on improving leaf nutrient content，and leaf nitrogen content increased by 16.11% compared with CK. Partial replacement of chemical fertilizer by organic fertilizer and its application combined with chemical fertilizer not only reduced the amount of chemical fertilizer，but also enhanced the quality of Jinggang tangerine as well as soil nutrient and enzyme activities. Considering the soil chemical properties，Jinggang tangerine yield and quality，70% of organic fertilizer substitution for chemical fertilizer is the best treatment.