Tillage and straw returning could change the stability of soil aggregates and physicalcharacteristics. The field positioning experiment was conducted to study the influence of tillage and straw returning depth on the stability of soil water-stable aggregates so as to provide a scientific basis for straw returning depth on dry meadowsoil. The results showed that stability of aggregates was affected by the proportion of macro-aggregates with grain size >2000 μm. The water-stable macro-aggregates with grain size >2000 μm in 0-20 cm soil layer was significantly decreased but contents of 53-250 μm micro-aggregates and clay silt particles <53 μm were increased with constantly tillage in fiveyears.Amongthem，clay silt particles <53μm with shallow tillage in 10-20 cm soil layer increased by 86.21% and14.65%，respectively，compared with no tillage and deeptillage， and deep tillage in 20-30 cmsoil layer increased by 113.82% and59.68%，respectively，compared with no tillage and shallow tillage (P<0.05).Deep tillage had a great impact on the reduction of 250-2000 μm particle size aggregates in 20-40 cm soillayer，which was19.43% and 20.57% onaverage，respectively，lower than no tillage and shallowtillage，straw mulching and straw shallow returning withsignificantdifferences( P<0.05).The stability of aggregates was reduced due to the disturbance of continuous deep tillage to thesubsoil，all of these can provide a scientific basis to improve the soil structural properties and the stability of aggregates and fertilize thesoil，and the depth of tillage and straw returning should be adjusted by means of rotation in future production.