The problem of replanting obstacle limits the healthy development of Angelica sinensisindustry.In order to establish an efficientplantingmethod，five planting methods were established inWeiyuancounty，Gansuprovince， includingA:pea -wheat-A.sinensis；B:pea -Mongolian astragalus-A.sinensis；C:pea -potato-A.sinensis；D:pea -A. sinensis-A.sinensis(control)；E:pea -fallow-A.sinensis.The gene sequences of ITS1 variation region of fungi in the rhizosphere soil under different cropping patterns were determined during A.sinensis harvest period by Illumina Hisqe 2500sequencing platform，and the effects of different cropping patterns on fungal communities and functional differences in the rhizosphere soil wereanalyzed.The results showed asfollows:(1)Compared to the controlD，the Chao1 index，Ace index and Shannon index forA，B，C and E treatments werelower，but the Simpson index washigher.(2)Soil fungi communities in the rhizosphere under five planting methods attached to 167 genera in 11phyla，inwhich Ascomycota，Mortierellomycota，Basidiomycota and Chytridiomycota were the dominant phyla and Mortierella and Tetracladium were the dominantgenera.(3)Redundancy analysis showed that availablepotassium，electricalconductivity，and pH value were the main factors affecting soil fungal community structure atphylumlevel，while organicmatter，pHvalue，and carbon dioxide respiration rate werethe main factors affecting soil fungal community structure at genuslevel.(4)FUNGuild prediction showed that the relative abundances of functional groups of plant pathogens and woody saprophytic fungi werehigher.Inconclusion，compared with continuous croppingA.sinensis，different crops rotation reduced the relativeabundance and diversity of fungal communities in the rhizospheresoil，which was conducive to the profitable development of soil fungal communities in the rhizosphere soil.