This research was conducted in order to accurately evaluate the effect of successive years of chemical fertilizer reduction with organic fertilizers on the yield of roasted tobacco and the comprehensive fertility quality of the planted tobaccosoil，and to investigate the chemical fertilizer reduction and organic replacement technology for the improvement of the planted tobacco soil quality and the synergy of green and high quality of roasted tobacco. In thisstudy，based on the data from the 7th year of the long-term locationaltrial，the quality of soil fertility was evaluated comprehensively using factoranalysis，correlation coefficientmethod，and Nemero index method for fourtreatments，including no fertilizer application(CK)，locally recommended conventional fertilizerapplication(100% chemicalfertilizer，CF)，20% reduction ofchemical fertilizer with organic fertilizer(OF)and with bio -organicfertilizer(BIO)，and random forest regression analysis was used to explore the degree of explanation of each individual soil fertility index on the yield of roasted tobacco. The results showed that under the condition of 20% reduction of chemical fertilizer for 7 consecutiveyears，the application of organic fertilizer and bio-organic fertilizer reduced the incidence and disease indexof green blight and black tibia of roastedtobacco， improved theeconomictraits，yield sustainability and stability of roastedtobacco，and improved the soil nutrient content andcomprehensive quality of soil fertility of planted tobacco. Random forest analysis showed that availablephosphorus，alkalinenitrogen，and total nitrogen of planted tobacco soils were the determinants of roasted tobaccoyield，with the explanation rates of19.66%，15.38% and12.07%，respectively. Under the three evaluationmethods，the IFI values of integrated fertility index of planted tobacco soils showed asBIO>OF>CF>CK，andBIO，OF treatments were increased by 23.03%-31.75% and 4.92%-7.94%，respectively，compared with CF treatment. Theintegrated fertilityindex(IFI)values of the three evaluation methods showed a significant or highly significant positive correlation with the relative yield of roastedtobacco，and the magnitude of the correlation coefficients were Nemero indexmethod(0.884)> factor analysis method(0.867)>correlation coefficientmethod(0.747)in order.Therefore，successive years of chemical fertilizer reduction with different organic fertilizers had a sustained effect on improving the overall fertility quality of the planted soil and the quality oftobaccoproduction，and the best effect was achieved with 20%chemical fertilizer reduction with bio-organic fertilizers.