In order to reduce the amount of chemicalfertilizer，give full play to the effect of organic fertilizer and effectively improve the quality of winegrapes，a field plot experiment with six treatments wasconducted，which were no fertilizercontrol(CK)，100% chemicalfertilizer(C 1)，100% organicfertilizer(O 1)，100% organic fertilizer +microbialagent(O 1B)，60% chemicalfertilizer +40% organicfertilizer(C 0.6O0.4)and 60% chemical fertilizer +40% organic fertilizer +microbialagent(C 0.6O0.4B). The contentsofN，P 2O5，K2O were the same in all fertilization treatments except CK. The effects of different fertilization treatments on soilnutrients，basic physicochemical properties and phenolic accumulation of grapes and wine were studied to analyze the correlation between soil environmental factors and quality indexes of grapes andwine，and the theoretical basis for scientific and efficient fertilization of wine grapes could be provided. The results showed that compared with CKtreatment，the soil organicmatter，totalnitrogen，total potassium，available potassium and soluble organic carbon treated by O1 and C0.6O0.4 treatments weresignificantly increased by 30.22% and70.65%，27.45% and31.37%，10.10% and 20.11% and 438.93% and491.60%， respectively. Compared with C1treatment，the soil organicmatter，totalnitrogen，totalpotassium，available potassium treated by O1B and C0.6O0.4B treatments weresignificantly increased by 101.30% and104.78%，27.45% and47.06%，13.58% and 23.12%and 2.23% and15.81%，respectively. Organic fertilizer with microbial agent treatment significantly improved the soil fertilityin vineyards. Compared withCK，the total solublesolids，reducingsugars，and sugar -acid ratio of grapes treated by C0.6O0.4B treatment wereincreased by4.93%，10.35% and49.78%，respectively；the alcohol of wine was increased by 6.67% and the total acid content wasdecreased by7.93%；the total phenol and total anthocyanin content of skins were increased by 26.98% and 19.56%，respectively；total phenols and total anthocyanins of wine wereincreased by 11.13% and56.98%，respectively. RDA and Spearman correlation analyses showed the variation of quality indicators of wine grapes and wine was closely related to soilenvironmental factors(organicmatter，nitratenitrogen，ammoniumnitrogen，availablepotassium，availablephosphorus)， the potential of co-application of microbial agent and organic fertilizers to improve the quality of wine grapes was confirmed. A comprehensive comparison showed that organic fertilizers and microbial agent could improve soil properties and the quality of winegrapes，thus improving the taste and color quality ofwine，with the best results of C 0.6O0.4B.