In order to provide theoretical basis forhigh yield，high quality and rational fertilization rate of Yun angelica inWeixiCounty，a field experiment of proprietary medicine cultivation of Yun angelica was conducted under weedproof cloth mulching. Seven treatments were setup，i.e. nofertilization(CK1)，conventional fertilization(CK2)，single chemicalfertilizer application(C)，single bio -organic fertilizer Ⅰapplication(B Ⅰ)，single bio-organic fertilizer Ⅱ application(BⅡ)，50% chemical fertilizer+bio-organic fertilizer Ⅰ(1/2C+BⅠ)，50% chemical fertilizer+bio-organic fertilizer Ⅱ (1/2C+BⅡ)，to explore the effects of different fertilization treatments onthe yieldcomponents，yield，quality and soil fertility of Yun angelica. The resultsshowed that:(1)Application of bio -organic fertilizer promoted an increase of main rootlength， rootdiameter，lateral rootnumber，fresh and dry weight perplant，and significantly increased both the fresh and dry yieldof Yunangelica( P<0.05). Compared to theCK1，all treatments with bio -organic fertilizer increased the fresh and dry yieldof Yun angelica by 41.50%-201.13% and35.00%-69.42%，respectively，of which 1/2C+B Ⅰ treatment had the highest freshyield and dry yield of Yunangelica，followed by the 1/2C+B Ⅱtreatment.(2)Application of bio -organic fertilizer increased the content of ferulicacid，volatile oil andpolysaccharide，reduced the total ashcontent，and effectively improved thequality of Yunangelica.(3)Application of bio -organic fertilizer significantly increased the content of soil organicmatter， alkaline-hydrolyzablenitrogen，available phosphorus and available potassium( P<0.05). Compared totheCK1，all bio -organic fertilizer treatment increased the contentof soil organicmatter，alkali -hydrolyzablenitrogen，available phosphorus and available potassium by11.77%-16.77%，5.64%-13.17%，15.74%-95.78% and26.71%-140.53%，respectively. Considering the yieldcomponents，yield，quality andsoil fertility of Yunangelica，the application of 1/2C+B Ⅰ treatment was the best with the high yield and high quality of Yun angelica in Weixi County under current experimental conditions.