Phosphorus played an important role in growth and development of plants. In order to obtain high-quality phosphate-solubilized growth-promotingbacteria，the rhizosphere soil of peach garden in Pinggudistrict，Beijing was used as the rawmaterial，and the strains with high phosphate -solubilizing and growth-promoting capacity were screened out. The strain that could produce phosphate-solubilizing ring was screened out by plate test and then cultured in liquid state. The maximum phosphate-solubilizing ability of the strain was determined by molybdenum-antimony colorimetricmethod，and the relationship between the phosphate-solubilizing ability of the strain and pH change was explored. At the sametime，the growth-promoting characteristics of the strain were studied by pot experiment. Three phosphate-solubilizingbacteria(JP01， JP03 andPG62)were isolated from the rhizosphere soil of peachtree，and preliminarily identified as Aspergillus niger， Pseudomonas aeruginosa and Corynebacterium pallidum.Among them， A. niger JP01 had excellent phosphate solubilizingability，its ability to dissolve Inorganic phosphorus was 112.52mg/L，the conversion amount of organic phosphorus was145.50mg/L，and the maximum phosphate solubilizing ability was95.74 mg/L. During the process ofculture，the pH of the culture solution corresponding to the phosphate-solubilizing bacteria decreased significantly. The pot experiment showed that A. nigercould increase the plantheight，stem diameter and underground dry weight of maizeseedlings，increase the total phosphorus content of the plants and had a certain effect on soil available phosphorus. The results of this study showed that A. niger met the technical specifications for the development of phosphate-solubilizing microbial fertilizers.