The soil integrated fertility index of tobacco-planting soil in Zunyi city was explored to provide theoretical support for tobacco planting management. The 3772 soil samples were collected from 168 towns in 13 districts (counties) in tobacco planting area of Zunyi city for chemical properties determination. The soil fertility of tobacco planting area was evaluated by fuzzy mathematics. Kriging interpolation method was used to draw the spatial distribution map of soil chemical properties and comprehensive fertility evaluation. The results showed as follows: (1) The contents of pH, organic matter, alkali-hydrolyzable nitrogen, available phosphorus and available potassium were significantly correlated. The contents of organic matter and alkali-hydrolyzable nitrogen reached high or higher level. The content of available phosphorus and available potassium was relatively high. The nutrients required for the growth of flue-cured tobacco were guaranteed. (2) The fertility index of tobacco soil had obvious spatial variation characteristics. The distribution of organic matter and alkali-hydrolyzable nitrogen were basically consistent, showing a great correlation. Organic matter, alkali-hydrolyzable nitrogen and available phosphorus were distributed in patches, while soil pH and available potassium were distributed in blocks. Among them, the regions with higher potassium content were basically consistent with the regions with lower soil pH. (3) The comprehensive evaluation score of tobacco soil fertility in Zunyi city was 0.73, and the variation coefficient was 16.8%. The total proportion of grade I to III was more than 93%. The soil fertility of tobacco planting area was positive, and the spatial variation was small. In conclusion, the tobacco soil fertility in Zunyi city can supply the demand of high-quality tobacco production, but the use of organic fertilizer and nitrogen fertilizer still needs to be strengthened in some counties, and improvement should depend on the comprehensive evaluation and spatial distribution of soil fertility in the tobacco planting area of Zunyi city.