Excessive use of inorganic phosphate fertilizers in agricultural production has triggered a number of problems such as soilslumping，and searching for alternatively sustainable phosphate fertility sources is necessary. Organic phosphate in soil exists mainly in form of phytate which plants cannot directly absorb and utilize. Releasing the phytate phosphorus using microbial phytase can provide an eco-friendly solution for sustainable phosphate fertility in agriculture. The method of present work was to add dual-domain β-propeller alkalinephytase(phyHT)from Bacillus sp. HJB17 to investigate its soil stability and test its effect on increasing the effective phosphorus content ofsoil，improving soil physical and chemical properties and promoting plant growth. The results showed that the soil microorganisms had little effect onphyHT，which was stable over a wide range oftemperature(20-40℃) andpH(6.0-9.0)for 12 hours and functions as an efficient catalyst in moist soils. After exogenous addition ofphyHT:(1)Soil phytate content was decreased by 17.00% and effective phosphorus content was increased by 22.52%；(2)Soil pH wasdecreased，the conductivity of soil wasincreased，CaCO 3 content was decreased by 3.55percentagepoint，and soil organicmatter，ammonium nitrogen and available potassium content wereincreased by10.54%， 18.38%and27.49%，respectively；(3)Wheatseedling growthlength，dry matteraccumulation，and total phosphorus content of shoot and root tissues were significantlyincreased.Therefore，exogenous addition of phyHT can promote soilphytatehydrolysis，increase soil effective phosphorus content and improve soil phosphoruseffectiveness，at the sametime， it can improve the physical and chemical properties of the soil to a certain extent and promote plant growth.