The effects of chemical fertilizer reduction combined with organic fertilizer onsoil enzymes andmicroorganisms，growth，yield and quality of cherry tomato werestudied，in order to clarify the response of cherry tomato to fertilizerreduction combined with organic fertilizer in yellow soil in southernChina，and to provide reference for high yield and quality production technology of cherry tomato and soil fertilizer cultivation. Taking cherry tomato‘feijiao’as theresearchobject， six treatments were set up on the basis of 5.0 t/hm² organic fertilizerapplication，namely CK(conventional fertilization)，T1(10% reduction of chemicalfertilizer)，T2(20% reduction of chemicalfertilizer)，T3(30% reduction of chemicalfertilizer)，T4(40% reduction of chemical fertilizer)and T5(50% reduction of chemical fertilizer). The results showed that the activities of acidphosphatase，sucrase and cellulase in soil were increasedby8.8%，14.6% and 31.9% with chemical fertilizer reduction combined withorganicfertilizer，respectively，and catalase activities were decreased by14.9%，while the activities of urease weredecreased by excessive reduction of chemicalfertilizer(T4，T5). The chemicalfertilizer reduced by10%-50%，the number of fruits per panicle increased by20.8%，18.3%，17.3%，12.9% and12.7%，respectively，and the yield increased by 10.6%，10.5%，8.4%，0.6%and0.2%，respectively. The quality of cherry tomato wasimproved，the soluble sugar content wasincreased by0.9%-8.2%，the soluble solid content was increased by 4.7%-11.3%，the vitamin C content wasincreased by1.3%-3.5%，the content of edible acid wasdecreased by8.5%-12.8%，and the sugar-acid ratio wasincreased by 1.0%-2.5%.Therefore，the most suitable nutrient management strategy was to reduce the amount of conventional chemical fertilizer by 20%-30% and apply moderate organic fertilizer by 5.0 t/hm².