解淀粉芽孢杆菌W48 对大蒜产量品质 及土壤质量提升效果研究
作者:
作者单位:

(1.江苏大学食品与生物工程学院,江苏 镇江 212013;2.江苏省农业科学院农产品质量安全与营养研究所,江苏 南京 210014)

作者简介:

熊雨洁(1999-),在读硕士研究生,研究方向为食品与生物技术。E-mail:1764576373@qq.com。

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基金项目:

基金项目:江苏中晚熟大蒜产业集群建设项目(13922204);江苏省农业科技自主创新资金[CX(22)3131]。


Effects of Bacillus amyloliquefaciens W48 on garlic yield and soil quality improvement
Author:
Affiliation:

(1.School of Food and BiologicalEngineering,JiangsuUniversity,Zhenjiang Jiangsu212013;2.Institute of Food SafetyandNutrition,Jiangsu Academy of AgriculturalSciences,Nanjing Jiangsu210014)

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    摘要:

    为明确微生物菌剂改善大蒜产地环境、提升产量品质的效果,利用常规施肥结合解淀粉芽孢杆菌W48 发酵液进行大蒜田土壤处理,测定大蒜产量和品质相关指标,监测土壤理化性质及生物转化相关酶活性变化,定量分析菌剂处理后土壤和大蒜中邻苯二甲酸酯类塑化剂(PAEs)含量差异。结果表明,W48 菌株具有分泌吲哚乙酸的能力,同时具备溶磷和产铁载体的潜力。施用W48 微生物菌剂显著增加了植物根际土壤有机质、碱解氮、有效磷和速效钾含量,改善根际区域营养环境,提高了土壤碱性磷酸酶、蔗糖酶和脲酶等生物转化相关酶活性,提高了土壤氮、磷元素利用效率;与对照相比,微生物菌剂处理的小区大蒜增产10%,可溶性糖和维生素C 含量等品质指标均有提高,其中可溶性糖显著增加了30%;邻苯二甲酸二正丁酯(DBP)和邻苯二甲酸二(2- 乙基)己酯(DEHP)是土壤和大蒜中PAEs 的主要检出类型,土壤中的平均浓度分别达到1874.05 和337.12 μg/kg,大蒜中几种PAEs 残留量普遍较高,DBP 最高为666.57 μg/kg,其次为邻苯二甲酸二异丁酯(DIBP)409.93 μg/kg 和邻苯二甲酸丁苄酯(BBP)433.65 μg/kg。菌剂对PAEs 具有显著的体外降解能力,土壤处理后能够显著降低土壤中PAEs 浓度,对DBP、DEHP 和邻苯二甲酸二甲酯(DMP)的去除率分别为25.5%、76.1% 和48.4%,但对大蒜中的PAEs 含量影响不显著。以上结果为大蒜产地土壤污染物修复及改良提供了技术支撑。

    Abstract:

    In order to clarify the effect of microbial agents on improving the environment of garlic producing area and improving the yield and quality ofgarlic,conventional fertilization combined with Bacillus amyloliquefaciens W48 fermentation broth was used to treat the soil in garlic field. The yield and quality related indexes of garlic weremeasured,andthe changes of soil physical and chemical properties and biotransformation related enzymes were monitored. The content of phthalate esterplasticizers(PAEs)in soil and garlic treated with microbial agents was quantitatively analyzed. The results showed that W48 strain has the ability to secrete indoleaceticacid,as well as the potential to dissolve phosphorus and produce iron carriers. The application of W48 microbial agent significantly increased the contentsof organicmatter,alkali -hydrolyzed nitrogen,available phosphorus and available potassium in the rhizospheresoil,improved the trophic environmentin therhizosphere,increased the activitiesof soil alkalinephosphatase,sucrase andurease,and improved soil nitrogen and phosphorus use efficiency. Compared with thecontrol,the garlic plots treated with microbial agents increased theyield by10%,and the content of soluble sugar and vitamin C wereimproved,among which the soluble sugar increased by 30%. Di-n-butyl phthalate(DBP)and di -2-ethyl hexyl phthalate(DEHP)were the main types of PAEs detected in soil andgarlic,with the average concentration of 1874.05and 337.12 μg/kg,respectively. The residual amount of PAEs in garlic was generally high. The highest DBP was666.57 μg/kg,followed by diisobutyl phthalate(DIBP)which was 409.93 μg/kgand butyl benzyl phthalate(BBP)which was433.65 μg/kg. W48 exhibited significant PAEs degradation ability invitro,andthe bacterial agent treatment could significantly reduce the concentration of PAEs insoil,but had no significant effect on the content of PAEs in garlic. Theremoval rates of DBP,DEHP and dimethyl phthalate(DMP) were25.5%,76.1% and48.4%, respectively. The results of this study can provide technical support for remediation and improvement of soil pollutants in garlic producing area.

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熊雨洁,宋立晓,王亚,生宏杰,余向阳,张雷刚.解淀粉芽孢杆菌W48 对大蒜产量品质 及土壤质量提升效果研究[J].中国土壤与肥料,2024,(2):89-96.

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  • 收稿日期:2023-02-14
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  • 录用日期:2023-06-15
  • 在线发布日期: 2024-05-22
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