In order to comprehensively analyze the characteristics of phosphorus pool in rhizosphere soil of flue-cured tobacco and its response to combined application of organic and inorganic fertilizers，this paper analyzed the differences of flue-cured tobacco yield，output value，proportion of different grades of flue-cured tobacco and phosphorus pool characteristics through field experiments.The results showed that the organic combined application of organic and inorganic fertilizers significantly increased the yield and output value of flue-cured tobacco，the proportion of superior tobacco and the content of different forms of phosphorus components.Compared with the conventional fertilization(treatment 2)，the yield，output value and the proportion of highgrade tobacco significantly increased by 30.00%，59.57% and 82.42% on average，respectively，with 20% reduction of chemical fertilizer combined with organic fertilizer(treatment 3)，biological organic fertilizer(treatment 4) and 30% reduction of chemical fertilizer combined with organic fertilizer and biological organic fertilizer(treatment 5)，while the contents of labile P，moderately labile P，sparingly and non-labile P significantly increased by 48.36%，67.15%，16.51% and 12.46% on average，respectively.The form of phosphorus components which most contributed to the tobacco yield was labile P，accounting for 68.23% of all phosphorus components，with Resin-Pi as the primary contribution factor，accounting for 68.70% of labile P components，followed by 17.30% of NaHCO3-Pi and 14.10% of NaHCO3-Po. What’s more，treatment 4 showed the highest content of each phosphorus component，which significantly increased the yield，output value and high grade leaves of flue-cured tobacco，while significantly reduced the middle and low grade leaves，reflecting the obvious yield advantage and weight loss and synergistic effect of chemical fertilizer reduction combined with bio-organic fertilizer.