Green manure is a high-quality organic fertilizer source，while the ecological adaptability of different species of green manure plants is distinct，and their positive effects on the yield of subsequent crops and soil fertility are highly influenced by environmental climate and soil conditions. Therefore，it is essential to screen suitable green manure plants according to local conditions. In this study，the yield and quality performance of flue-cured tobacco and soil fertility were analyzed in four fertilization treatments，including commercial organic fertilizer (CK)，returning Vicia villosa Roth var (CV)，returning Lolium perenne L.(CL)and returning Brassica napus L.(CB). The results showed that compared with CK，the CV，CL，and CB treatments reduced the application of composite base fertilizers by 150，75 and 75 kg/hm²，respectively，and replaced 1800 kg/hm² of commercial organic fertilizer. The three types of green fertilizers increased the yield，output value，and output-input ratio of flue-cured tobacco after rolling，ranking as CV>CB>CL. The yield of flue-cured tobacco increased by 28.79%(CV)，16.10%(CB)，and 0.95%(CL)，respectively. The contents of total N，P，K，Cl and nicotine and the proportion of high-quality tobacco in flue-cured tobacco treated with green manure were higher than those of conventional fertilization，and the increases were 10.0%-17.78%(total N)，8.33%-25.0%(total P)，16.56%-24.84%(total K)and 34.20%-61.47%(nicotine)，respectively. Among them，the CV treatment had the best effect on improving the quality of tobacco leaves. In addition，planting green manure increased the content of soil organic matter，available nitrogen，phosphorus，and potassium，and increased the activities of sucrase，urease，and protease. Compared to Brassica napus L. and Lolium perenne L.，Vicia villosa Roth var was the best in the Zunyi tobacco growing area of Guizhou，and its effects on the improvement of yield，quality，and economic benefits of subsequent flue-cured tobacco，as well as the improvement of soil available nutrients and enzyme activity were better after rolling. Therefore，Vicia villosa Roth var is a more suitable green fertilizer variety for planting in the northern Guizhou tobacco region.