With the aim of efficient selenium-enriched production of millet in selenium-deficient soil region，the soil selenium absorption capacity of millet in selenium-deficient soil(cinnamon soil，with selenium content of 0.266 mg/kg)and the absorption efficiency of selenium by spraying exogenous selenium and synergistic preparations were studied. Taking ‘Jinggu No.2’，‘Jinggu No.3’ and ‘Jinggu No.4’ millet as test materials，four selenium levels(0，11.25，15.00，22.50 g/hm²)and two synergists(18.75 L/hm² humic acid and 0.19 L/hm² organosilicon)were set up. The field experiment was conducted in a randomized block design to study the interaction between millet varieties and selenium enrichment under the condition of exogenous selenium (Na2SeO3)application and the improvement of selenium enrichment ability by synergistic preparation. By measuring the grain selenium content，grain selenium utilization efficiency and intensification index of millet，the effects of different synergists and selenium on the efficiency of millet selenium enrichment were compared，which would be providing a reference for optimizing the production of high value-added selenium-rich agricultural products in production. The results showed that the grain selenium content and grain selenium bioconcentration index of ‘Jinggu No.2’ were the highest in CK treatment，and ‘Jinggu No.2’ was selected as the variety with strong soil selenium enrichment ability. Among the Se treatments，the grain selenium content，selenium utilization efficiency and selenium bioaccumulation factor of ‘Jinggu No.4’ were the highest，and ‘Jinggu No.4’ was selected as the variety with strong exogenous selenium enrichment ability. Compared with the synergistic preparation of selenium，spraying humic acid synergist increased the selenium content，selenium utilization efficiency and strengthening index of millet grains significantly. Spraying humic acid synergist，the grain selenium content of ‘Jinggu No.4’ was increased by 30.37% compared to Se2 treatment， and the grain selenium content of ‘Jinggu No.2’ and ‘Jinggu No.3’was increased by 41.50% and 16.81% compared to Se3 treatment，respectively. ‘Jinggu No.4’ was the suitable variety in the production of selenium-enriched millet，and humic acid synergist and 15.00 g/hm² Na2SeO3 were sprayed to improve the selenium-enriched efficiency of different varieties of millet. Among them，‘Jinggu No.2’ and ‘Jinggu No.3’ can be proved in reducing the amount of sodium selenite by 1/3，reaching the goal of selenium-enrichment in grain.