Qianjiang tobacco area is a high-altitude rainy tobacco area with low temperature before and after transplanting，which is not conducive to the growth and development of tobacco seedlings. To improve this situation，a rooting agent containing humic acid water-soluble fertilizer was applied in a concentration gradient in 2022. After transplanting the tobacco seedlings，it was divided into four concentration treatments，namely：CK（0 mg/L），T1（60 mg/L），T2（90 mg/L）， T3（120 mg/L）. Each plot was irrigated and sprayed with 50 L solution according to the concentration of T1，T2 and T3 treatments. The effects of humic acid on the yield and quality of flue-cured tobacco variety of Yunyan 87 were investigated from agronomic traits，appearance quality，chemical composition and economic traits of flue-cured tobacco leaves. T2 and T3 treatments were better than T1 treatment and CK in all aspects，while the agronomic traits and field growth potential of T2 and T3 treatments were basically the same，the plant height and leaf area of T3 treatment was slightly higher than those of T2 treatment；the dry fresh ratio of all parts of the tobacco of T2 treatment was better. In the chemical composition of fluecured tobacco leaves，the C3F grade of tobacco leaves of T2 treatment was the best，and the nicotine and nitrogen contents of T3 treatment in B2F grade tobacco leaves were slightly higher than those of T2 treatment. The tobacco evaluation of smoking quality of T2 and T3 treatments was not much different，the aroma and aroma amount of T2 treatment were slightly more； In terms of economic value，the yield and output value of T2 treatment were the best. After comparative analysis，it was concluded that T2 treatment was the best concentration treatment.