青藏高原野生宽叶羌活根际土壤微环境变化 对光合作用的影响
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(青海大学医学院/ 青海省糖脂代谢疾病防控中医药重点实验室,青海 西宁 810001)

作者简介:

胥生荣(1985-),副教授,博士,研究方向为药用植物生理生态。E-mail:xushengrong888@163.com。

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基金项目:青海省基础研究计划项目(2024-ZJ-951);青海大学2021 年度青年科研基金项目(2021-QYY-6)。


Effects of soil microenvironmental changes on photosynthesis in the wild notopterygium forbesii rhizosphere in the Qinghai-Tibet Plateau
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(Medical College/QinghaiProvincial Key Laboratory of Traditional Chinese Medicine Research for Glucolipid MetabolicDiseases,QinghaiUniversity,Xining Qinghai810001)

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    摘要:

    为进一步研究青藏高原野生宽叶羌活药材形成对土壤环境的响应特性,明确土壤环境在宽叶羌活不同生长年限的差异,以不同生长年限野生宽叶羌活根际土壤为试验材料,研究根际土壤酶活性和有机碳含量的变化规律。结果表明,宽叶羌活对根围土壤孔隙度的影响主要在土层0 ~ 40 cm 深处,5 年生地块平均土壤孔隙度减小至对照的88.56%,对土层50 ~ 100 cm 处影响较小。土壤含水量在土层0 ~ 50 cm 处变化显著,前3 年根围土壤含水量逐年增大,达到对照的105.59%,土层60 cm 以下的土壤水分含量变化规律一致且差异不显著。土壤有机碳含量逐渐下降,从对照到5 年生地块,土壤总有机碳含量降低到原来的41.75%,其中,可溶性有机碳含量降低到原来的51.78%,颗粒有机碳含量降低到原来的18.30%,活性有机碳含量降低到原来的24.42%,易氧化有机碳含量降低到原来的21.76%,微生物量碳含量降低到原来的47.31%;可溶性有机碳含量在3 年生和5 年生土壤间差异不显著,活性有机碳和易氧化有机碳含量在2 年生和3 年生土壤间差异不显著。随着生长年限增长,土壤脲酶、多酚氧化酶和过氧化氢酶活性呈先降低后升高的变化趋势,土壤脱氢酶和蔗糖酶活性没有明显变化趋势,土壤磷酸酶活性呈显著升高的变化趋势。随着株龄增大,叶片表观量子效率显著减小,光补偿点、光饱和点和暗呼吸速率均显著增大;叶片的原初光能转换效率、光化学淬灭系数和光合电子传递效率均表现不同程度的减小,非光化学淬灭系数表现显著增大,综合分析表明,随着生长年限的增加,土壤理化活性和部分土壤酶及有机碳含量降低,土壤微环境对根系生物活性的影响作用增大,根系生存环境的变化影响植株对土壤水分和营养物质的吸收,植株叶片同化效率降低,进而影响宽叶羌活药材质量和品质形成。

    Abstract:

    In order to further study the response characteristics of wild notopterygium forbesii to thesoil environment,and clarify the difference of soil environments in differentgrowth years,the soil enzyme activity and organic carbon content of the rhizosphere soil were monitored for wild notopterygium forbesii for three treatments with different continuous growth years(2,3 and 5 years). With the increaseof growthyears,the influence on soil porosity wasmainly in the depth of 0-40cm, the average soil porosity of 5-year old plots was decreased to 88.56% of thecontrol,and the influence on the soil porosity at 50-100 cm soil layer was small. The soil moisture content in the 0 -50 cm soil layer changedsignificantly,that in the root circumference was increased year by year inthe first 3 years,reaching 105.59% of thecontrol,and the soil moisture content in the soil below 60 cm soil layer had the same change law and the difference was not significant. Soil organic carbon content wasdecreased gradually,from the control to the five-year-old plots,the total soil organiccarbon(TOC)contentwas reduced to41.75% of theoriginal,and dissolved organiccarbon(DOC)content was decreasedto51.78%,particulateorganiccarbon(POC)content wasdecreased to18.30%,active organiccarbon(LFOC)content wasdecreased tO24.42%, easily oxidizedorganiccarbon(ROC)content was decreased tO21.76%,and microbial biomasscarbon(MBC)content was decreased to 47.31% of the original. There was no significant difference in DOC content between 3-year and 5-year oldsoils,and no significant difference in LFOC and ROC contents between 2-year and 3-year old soils. The activities ofurease, polyphenol oxidase and catalase in soil were first decreased and thenincreased,and the activities of dehydrogenase and sucrase in soil had no obvious changetrend,and the activity of phosphoric acid in soil was increased significantly. With the ageof plants,the Leaf apparent quantumefficiency(AQP)was significantly wasdecreased,and the light compensationpoint(LCP),light saturationpoint(LSP)and darkrespirationrate(Rd)were all significantly increased. The primary light energy conversionefficiency(Fv/Fm),photochemical quenchingcoefficient(qP)and photosynthetic electrontransferefficiency(φPSII)of leaves were all decreased tovarying degrees,and the non-photochemicalquenching(NPQ) coefficient was increased significantly. These results suggest that soil physical and chemical activity and some soil enzyme and organic carbon content weredecreased with the increase of growthyears,the influence of soil microenvironment on root biological activity wasincreased,and the change of root living environment affected the absorption of soil water and nutrientsby plants,and the plant leaf assimilation efficiency wasreduced,further affected the quality and quality formation of live medicinal materials of notopterygium root.

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胥生荣,马瑞丽,田国鹏,祁焕华,徐吉媛,韩梅花.青藏高原野生宽叶羌活根际土壤微环境变化 对光合作用的影响[J].中国土壤与肥料,2024,(7):184-191.

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  • 收稿日期:2023-08-21
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  • 录用日期:2023-09-13
  • 在线发布日期: 2024-09-30
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