In this study，under the condition of sufficient supply of nitrogen，phosphorus and potassium，the combination of basal application (R) and foliar spraying (L)was used to set up eight different fertilization treatments，including CK，Zn (L)，Fe(L)，Zn(R)，Zn(R)+Fe(L)，Se(R)，Se(R)+Zn(L)，Se(R)+Fe(L)，to investigate the effects of iron，zinc and selenium combined application on tomato growth，antioxidant capacity and glucose metabolism. The results showed that:1)Compared with CK，stem diameter of Zn(R)treatment was significantly increased by 11.24%，and internode length of Zn(R)+Fe(L)treatment was significantly increased by 30.13%. 2)Zn(R)+Fe(L)treatment significantly increased chlorophyll a and chlorophyll b by 26.13% and 41.03%，respectively. Fe(L)，Zn(R)+Fe(L)，Se(R)+Zn(L)treatments significantly increased net photosynthetic rate by 50.28%，70.92%，29.81%，respectively. 3)Single application and combined application of iron，zinc and selenium could increase the content of active substances in fruits. Lycopene and vitamin C in the Se(R)+Zn(L)treatment significantly increased by 1.04 and 1.15 times，respectively，and the content of total phenols and flavonoids significantly increased by 39.19% and 37.84%，respectively. Se(R)+Zn(L)and Se(R)+Fe(L)treatments significantly increased the activities of antioxidant enzymes and enhanced the antioxidant capacity of fruits. 4)Except for Se(R)treatment， iron，zinc and selenium application alone and combined application significantly increased reducing sugar and sucrose contents in tomato fruits. iron，zinc and selenium applied alone as well as in combination were able to regulate sugar metabolism related enzyme activities，in which the activity of sucrose synthase in tomato fruits was significantly increased by 79.67% under Zn(L)treatment，and the activity of sucrose phosphate synthase in tomato fruits was significantly increased by 1.09 and 1.17 times under Zn(L)and Zn(R)+Fe(L)treatments，respectively.