The effects of different nitrogen fertilizer management on the soil available nitrogen content and rice yield under rape straw incorporation were studied，which could provide a theoretical basis for nitrogen fertilizer management of rice under the total rape straw incorporation. Using Yuanliangyou 968 as the experimental material，an experiment was conducted in Yangzhou，Jiangsu Province in 2022. Five treatments were set up，namely rape straw incorporation without fertilization(SN0)，rape straw incorporation with nitrogen fertilizer(SN1，base fertilizer∶tillering fertilizer∶ear fertilizer=2.2∶2.2∶5.6)，rape straw incorporation with nitrogen fertilizer(SN2，base fertilizer∶tillering fertilizer∶ear fertilizer=2.5∶2.5∶5)，rape straw incorporation with nitrogen fertilizer(SN3，base fertilizer∶tillering fertilizer:ear fertilizer=3∶3∶4)，rape straw incorporation with nitrogen fertilizer(SN4，base fertilizer∶tillering fertilizer∶ear fertilizer=3.5∶3.5∶3). Soil ammonium nitrogen，nitrate nitrogen，soluble total nitrogen，urease，plant nitrogen content，and yield were measured at the seedling，heading，and maturity stages. The results showed that compared with SN0 treatment，the rice yield of SN1，SN2，SN3 and SN4 treatments increased by 15.73%，43.78%，68.05% and 43.78%，respectively. Under rape straw incorporation at seedling stage，the content of ammonium nitrogen increased significantly with the increase of the proportion of base fertilizer，and the activity of urease decreased. At heading stage，soil ammonium nitrogen and urease activity under SN3 treatment was significantly higher than other treatments，and soil nitrate nitrogen was significantly lower than other treatments. Compared with SN0 treatment，the aboveground nitrogen uptake in rice under SN1，SN3 and SN4 treatments was significantly increased by 37.34%，62.37%，57.65% and 19.47%，respectively. Under rape straw incorporation，increasing the proportion of base fertilizer had positive effects on rice yield and nitrogen uptake. When basal fertilizer∶tillering fertilizer∶ear fertilizer=3∶3∶4，both yield and nitrogen fertilizer utilization were at a high level.