In order to solve the problems of unbalanced nutrient supply and low fertilizer utilization in the growth process during long-season cultivation and improve the absorption and utilization of soil nutrients of tomato，the application effects of microbial agents and corn protein ferment under long-season cultivation were studied using tomato cultivar ‘Saint Laurent 3689’ as the test material.A total of four treatments were set up:normal management(CK)，CK+microbial agent(T1)，CK+corn protein ferment(T2)and CK+combined application of microbial agent and corn protein ferment(T3).The soil physicochemical properties，plant dry matter weight，nutrient content and accumulation，nutrient utilization efficiency，and fruit quality were measured for each treatment.The results showed that on the basis of CK，single application of microbial agent or corn protein ferment could promote plant growth and nutrient utilization，and the combination effect of them(T3 treatment)was better.Compared with CK，the soil available nitrogen，available phosphorus，and available potassium content increased by 24.9%，37.3%，and 22.2%，respectively.The dry weight of the plant increased by 8.4%，and the content and accumulation of nitrogen，phosphorus，and potassium in the plant increased by 15.3%，6.1%，9.7% and 19.0%，15.7%，18.9%，respectively.The utilization efficiency of nitrogen，phosphorus and potassium fertilizer increased by 64.9%，47.3% and 33.6%，respectively.The fruit quality of the ninth cluster was the best under T3 treatment，the contents of soluble solids，soluble sugars，vitamin C，and lycopene increased by 6.9%，13.1%，12.2% and 16.0%，respectively.In conclusion，the combination of microbial agents and corn protein ferment could increase the soil available nitrogen，available phosphorus，and available potassium content of long-season tomato cultivation，promote nutrient absorption by plants，improve nutrient utilization efficiency，increase plant dry weight，and improve fruit quality.Therefore，the combination of microbial agents and corn protein ferment can be applied in tomato production.