In order to ameliorate the deteriorative soil properties in chemical and biological aspects in the fields where Chinese herbal medicine plants grow on-site for years with long growth period and robust secondary metabolism，a field experiment was conducted in Anguo city，Hebei province，the hometown of Chinese herbal medicine (CHM). Three different treatments were designed，including farmer’s conventional fertilization pattern (CK)，lime application (Lime)，and compound conditioner application (Com) (complex of lime，gypsum，humic acid and Bacillus). The improvement effects of the soil conditioner on the chemical and microbiological properties of the soil where CHM were continuously planted for years，as well as the yield and quality of Bupleurum marginatum (BM) were examined. Results showed that the soil conditioners enhanced the yield and saponin content of BM by 33.8%-41.9% and 16.0%-38.3%，respectively. The conditioner had a positive effect on inhibiting the decrease of rhizosphere soil pH. Soil conditioners improved soil nutrient status，promoted the preservation of nitrogen，phosphorus and potassium nutrients in the soil，and facilitated plant absorption. Treatments with conditioners showed enhancement on soil enzyme activities，including sucrase and polyphenol oxidase，which indicating the improvement of the soil quality and health status. Treatments with the conditioners exhibited the improvement on the microbial community with a decrease in the compositional proportion of pathogenic fungi，including Mortierella，Talaromyces，Plectosphaerella，Colletotrichum，while an increase in beneficial fungi such as Coniothyrium，Alternaria. This study demonstrated that the compound soil conditioner containing chemical components and microbial bacteria (Bacillus complex) could apply as a partial solution for reducing soil barriers resulted from continuous cropping of Chinese medicine plants. The compound soil conditioners could be recommended as an option for improving soil quality and increasing the yield and quality of Chinese herbal medicine.