In order to clarify the application effect of biogas slurry fertilizer on substrate cultivated vegetables，the celery (Apium graveolens L.)and tomato(Solanum lycopersicum L.)were used as test materials in this experiment. Irrigated water was used as control(CK)，four treatments were set up，including commercial water-soluble fertilizer solution(WSF)，biogas slurry solution(BS)，balanced biogas slurry nutrient solution 1(BSF1)and balanced biogas slurry nutrient solution 2(BSF2). The effects of different treatments on the growth，SPAD，nutrient absorption and utilization of vegetables were analyzed. The results showed that: (1) WSF，BSF1 and BSF2 treatments increased the growth rate (GV) and biomass (BW) of the two vegetables. Compared with CK，the GV of celery treated with WSF，BSF1 and BSF2 treatments were increased by 94.74%，110.53% and 102.63%，respectively，and the GV of tomato were increased by 18.27%，49.04% and 29.81%，respectively. The dry weight (DW) of celery were increased by 55.98%，85.42% and 73.46%，respectively. DW of tomato were increased by 82.58%，137.33% and 135.58%，respectively. There was no significant difference between CK and BS treatment in FW and DW. (2) WSF，BSF1 and BSF2 treatments significantly increased the total nitrogen (TN) content，nutrient accumulation (NAA) and fertilizer physiological efficiency (PEF) of substrate cultivated vegetables. Compared with WSF treatment，BS treatment significantly reduced the fertilizer agronomic efficiency (AEF)，fertilizer use efficiency (UEF) and fertilizer contribution rate (CRF) of the two vegetables，while BSF1 and BSF2 treatments increased the AEF，UEF and CRF of tomato by 78.24%，135.11%，36.60%，and 85.63%，127.56%，41.83%，respectively. (3) Using the average weighting method，through the comprehensive analysis of five indexes，including GV，BW，SPAD，UEF and AEF，the application effects of different treatments on substrate vegetables were BSF1>BSF2> WSF>BS. In summary，the biogas slurry balanced nutrient solution could increase the TN content，NAA and PEF by increasing the GV and BW of celery and tomato cultivated on substrate，and ultimately increased AEF，UEF and CRF，and BSF1 showed the best effect.