The degraded alpine meadow in the source region of the Yellow River was taken as the research object，and bacterial manure and sheep manure were selected as the experimental materials to explore the changes of soil nutrients and enzyme activities during ecological restoration，in order to provide theoretical basis for ecological restoration of degraded alpine meadow in patches. The results showed that different fertilization treatment changed soil capacity，soil water content and pH. Both single sheep plate manure and single bacterial fertilizer treatment increased the content of soil organic carbon，total nitrogen，total phosphorus，nitrate nitrogen，ammonia nitrogen and available phosphorus，and there was no significant difference between them. But the combined application of sheep plate manure and bacterial fertilizer significantly improved the nutrient content of the above soil. The improvement of the improvement of the soil enzyme activity of combined application of sheep plate manure and bacterial fertilizer was significantly better than that of single sheep plate manure or single bacterial fertilizer treatment. Related analysis showed that there were significant correlation between soil enzyme activity and soil factors，of which soil enzyme activity was significantly affected by nitrate nitrogen. According to RDA analysis，under different fertilization treatment，ammonia nitrogen and pH in the soil were environmental factors affecting soil enzyme activity. pH value was positively correlated with soil capacity，and negatively with the water content. Generally speaking，in the ecological restoration of the degraded alpine meadow in the source region of the Yellow River，the combined application of 1500 kg sheep manure and 250 kg bacterial fertilizer per hectare showed the best effect.