To investigate the effects of plant growth promoting rhizobacteria(PGPR)on maize growth and soil nitrous oxide(N2O)emission，the effects of control，Pseudomonas stutzeri NRCB010 and Bacillus velezensis NRCB026 on maize seedling growth and soil N2O emission were compared based on the greenhouse potting experiment and 16S rDNA amplified high-throughput sequencing.Compared with the control，inoculation with NRCB010 and NRCB026 significantly promoted the growth of maize seedlings，increased dry weight by 41% and 35%，reduced cumulative soil N2O emissions by 54.6% and 21.6%，respectively；at harvest，the relative abundance of Sphingomonas and Flavisolibacter in the dominant genera of soil bacteria in NRCB010 treatment increased by 12% and 0.5%，respectively，compared with the control；and the relative abundance of Sphingomonas and Flavisolibacter in NRCB026 treatment increased by 11% and 0.7%，respectively.FAPROTAX functional prediction results indicated that the abundance of microbial genes for soil denitrification and nitrate reduction was higher in NRCB010 and NRCB026 treatments than in the control group.Pearson correlation and redundancy analyses further indicated that pH was the main factor influencing PGPR to reduce soil N2O emissions.NRCB010 and NRCB026 reduced soil N2O emissions by increasing the abundance and activity of denitrifying microorganisms in maize rhizosphere soil.The results of this study provided a technical reference for the application of PGPR to reduce soil N2O emissions in agricultural fields.