To comprehensively evaluate the effects of organic fertilizer nitrogen(N) replacing chemical N on tea yield quality and soil fertility，and to guide the scientific application of organic fertilizer in tea gardens，a field experiment was conducted in Meitan County，Guizhou Province，with single application of chemical fertilizer(ON0) as the control，and organic fertilizer N replacing 20%(ON20)，40%(ON40)，60%(ON60)，80%(ON80) and 100%(ON100) of chemical N fertilizer，and the changes in the yield and quality，and soil fertility were analyzed for each treatment.The results showed that with the increase of the proportion of organic fertilizer nitrogen substitution，the tea yield showed a trend of increasing and then decreasing，among which the ON20 treatment was the highest with an increase of 21.53%，compared to the ON0 treatment.The replacement of chemical fertilizer N with organic fertilizer N significantly increased the total free amino acids of tea leaves，reduced the phenol-ammonia ratio of tea leaves，and improved the quality of tea leaves.Compared with the single application of chemical fertilizer，the replacement of chemical fertilizer N by organic fertilizer N could improve soil pH and organic matter，and reduce soil ammonium nitrogen and microbial biomass nitrogen.Compared with the single application of chemical fertilizer treatment，under the condition of replacing 20% of fertilizer N with organic fertilizer N，the nitrate N，alkaline dissolved N and total soluble N of tea plantation soil were increased by 21.35%，3.13% and 10.73%，respectively.In this study，replacing 20% of chemical fertilizer N with organic fertilizer N resulted in an improvement in tea yield and quality，as well as an increase in soil pH，organic matter，and nitrogen content，which could be an optimal organic fertilizer combined application with chemical N mode.