The effects of different fertilization measures on soil and tea yield and quality of karst tea garden were explored in order to establish a reasonable fertilization mode.Field experiments were carried out in centralized continuous tea garden in karst area of Bijie City，Guizhou Province.Control group(CK)and four different fertilization measures were set up:application of rapeseed cake fertilizer(T1)，application of biological fertilizer(T2)，application of organic fertilizer(T3)，intercropping with green manure and white trefoil(T4).The effects of different fertilization measures on soil nutrients，soil enzyme activities，tea yield，mineral elements and tea quality elements were studied. Compared with CK，the largest increase in soil nutrients in each treatment was exchangeable calcium(3.46 cmol/kg)in T1，pH(5.92)，total phosphorus(0.85 g/kg)，total potassium(10.10 g/kg)，available manganese(37.35 mg/kg)，available copper(3.31 mg/kg)，and exchangeable magnesium(0.60 mg/kg)in T2.The organic matter(60.54 g/kg)，total nitrogen(3.91 g/kg)，alkali-hydrolyzed nitrogen(286.75 mg/kg)，available phosphorus(8.50 mg/kg)，available potassium(126.58 mg/kg)，and available iron(4.65 mg/kg)in T3.T2 and T3 had better effects on soil nutrient enhancement.The largest increase in soil enzyme activity in each treatment was urease [1.05 mg/(g·d)] and acid phosphatase [7.18 mg/(g·d)] in T3，catalase(3.03 mL/g)in T4，and sucrase [53.51 mg/(g·d)] in T1.The effects of each treatment on catalase were not significant.Among all treatments，T3(1907 kg/hm²)showed the largest increase in tea yield. N(20.14 g/kg)，P(3.15 g/kg)，Mg(0.97 g/kg)，Mn(478.47 mg/kg)and Cu(12.04 mg/kg)in T3 and K(22.06 g/kg)，Ca(1.53 g/kg)，Al(8.56 g/kg)，Fe(352.72 mg/kg)，Zn(43.60 mg/kg)in T2 were the maximum increases in mineral elements of tea，respectively.The highest improvement of tea quality was amino acid(14.40 mg/kg)，caffeine(24.78 mg/kg)，water extract(40.40%)，catechin(546.60 g/kg)in T3 and tea polyphenols(131.1 mg/kg)in T2.The four fertilization measures all improved soil fertility and tea quality to a certain extent.The improvement effects of application of biological fertilizer and organic fertilizer on soil nutrients and tea mineral elements were better.The improvement effects of application of organic fertilizer on soil enzyme activity and tea quality were the best，followed by biological fertilizer.The contents of soil organic matter，alkali-hydrolyzed nitrogen，available phosphorus，available potassium and phosphatase were significantly positively correlated with the nutritional indexes of most tea leaves.A total of 4 principal components were extracted from the analysis of principal components.The comprehensive evaluation ranking of different fertilization treatment benefits was as follows:application of organic fertilizer > application of biological bacterial fertilizer > application of rapeseed cake fertilizer > application of green fertilizer and white clover.