秸秆还田下连作对黄瓜土壤细菌群落结构和多样性的影响
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(辽宁省微生物科学研究院/辽宁省微生物菌种选育重点实验室,辽宁 朝阳 122000)

作者简介:

李杨(1982-),硕士研究生,主要从事土壤微生物生态与调控等方面研究工作。E-mail:liyang_0223@163.com。

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基金项目:

辽宁省重点研发计划项目(2022020386-JH2/1013);辽宁省国际科技合作计划项目(2023030481-JH2/107)。


Effects of continuous cropping on soil bacterial community structure and diversity of cucumber under straw returning
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(Microbial Research Institute of Liaoning Province / Key Laboratory of Selective Breeding of Microbial Culture Liaoning Province ,Chaoyang Liaoning 122000)

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    摘要:

    为研究秸秆还田下设施黄瓜连作不同年限对土壤细菌群落结构和多样性的影响,探索秸秆还田下连作土壤修复的微生物学机理,以玉米秸秆还田下设施黄瓜连作1、3、5、10和15年的根区土壤为研究对象,利用高通量测序和实时定量PCR技术解析土壤细菌群落组成、群落多样性和潜在功能的变化特征,分析其变化规律。秸秆还田下连作土壤有机质和全氮含量提高,铵态氮含量降低,土壤pH呈先降后升的趋势,电导率呈逐年上升的趋势,碱性磷酸酶和蔗糖酶活性升高。不同处理土壤细菌的基因拷贝数为2.98×109~7.81×109 个/g,秸秆还田下连作提高了土壤细菌的丰度。放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、厚壁菌门(Firmicutes)4个菌门相对丰度稳定在75%以上,随着连作年限延长绿弯菌门和酸杆菌门(Acidobacteria)的相对丰度均有所增加,厚壁菌门的相对丰度有所减少。多样性分析表明,秸秆还田下土壤中细菌的多样性无显著变化(P>0.05)。主坐标分析显示,连作可以影响细菌群落结构,不同处理细菌群落结构均差异极显著(P<0.01)。FAPROTAX功能预测显示,化能异养型和好氧化能异养型为主要类型。共现网络分析中,秸秆还田下连作提升了属水平网络的复杂度和稳定性。冗余分析结果显示,全磷对土壤细菌群落结构的影响最为显著(F=3.5,P=0.002),解释了21.3%的群落变化,贡献率依次为全磷>铵态氮>有效磷>pH>速效钾>电导率>全氮>有机质;细菌群落对脲酶的影响最显著(F=4.6,P=0.002),解释了26.3%的群落变化,对各酶活的影响顺序为脲酶>过氧化氢酶>蔗糖酶>多酚氧化酶>碱性磷酸酶。因此,秸秆还田对稳定连作土壤细菌多样性具有积极作用及在缓解设施作物连作障碍中的有益效果,土壤全磷是影响土壤细菌群落结构变化的主效环境因子,细菌群落的改变对土壤脲酶活性影响最大。

    Abstract:

    The experiment studied the effects of different years of continuous cropping on soil bacterial community structure and diversity in facility cucumber field under straw returning,so as to explore the microbial mechanism of soil restoration of continuous cropping soil.Five different rhizosphere soil treatments were sampled,including continuous corn-returning for 1,3,5,10 and 15 years.High-throughput sequencing and quantitative PCR were used to analyze the characteristics of soil bacterial community composition,community diversity and potential functions,as well as the change patterns.Continuous cropping under straw returning increased the contents of organic matter,total nitrogen,reduced alkali-hydrolyzale nitrogen.Soil pH decreased first and then increased.With the increase of cropping years,electrical conductivity increased.The activities of alkaline phosphatase and sucrase increased.The gene copy numbers of soil bacteria in different treatments ranged from 2.98×109 to 7.81×109 copies·g-1 soil.It was found that continuous cropping under straw returning increased the abundance of soil bacteria.The relative abundance of Actinobacteria,Proteobacteria,Chloroflexi and Firmicutes was stable at more than 75%.With the increase of cropping years,the relative abundance of Chlorocurvula and Acidobacteria increased,while the relative abundance of Firmicutes decreased.Diversity analysis showed that there were no significant changes in bacteria diversity(P>0.05).The principal coordinate analysis showed that the differences in bacterial community structure among treatments were highly significant(P<0.01),which indicated continuous cropping could affect the bacterial community structure.FAPROTAX function prediction showed that chemoheterotrophy and aerobic_chemoheterotrophy were the main types.The co-occurrence network analysis showed that the complexity and stability of the genus level enhanced after continuous cropping.Redundancy analysis showed that total phosphorus had greatest effect on the soil bacterial community structure (F=3.5,P=0.002),explaining 21.3% of the community changes.The order of contribution was total phosphorus>alkali-hydrolyzale nitrogen>available phosphorus>pH>available potassium>electrical conductivity>total nitrogen>organic matter.The bacterial community had the greatest effect on urease(F=4.6,P=0.002),accounting for 26.3% of the community changes.The order of effect on enzyme activity was urease>catalase>sucrase>polyphenol oxidase>alkaline phosphatase.The positive effect of straw return on stabilizing soil bacterial diversity in continuous cropping and the beneficial effect in mitigating continuous cropping barriers in facility crops were confirmed.Total phosphorus was the main effective environmental factor influencing the changes of soil bacterial community structure.The change of bacterial community had the greatest effect on soil urease activity.

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李杨,宋立群,敖静,刘晓辉,高晓梅,孙玉禄,朱巍巍.秸秆还田下连作对黄瓜土壤细菌群落结构和多样性的影响[J].中国土壤与肥料,2025,(2):65-75.

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  • 收稿日期:2024-06-11
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  • 录用日期:2024-08-02
  • 在线发布日期: 2025-04-24
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