Continuous cropping obstacle is a major bottleneck affecting the cultivation of the precious herb Sanqi(Panax notoginseng)，and changes in fungal communities in the soil are the key factor leading to the continuous croping obstacle.In this study，a pot experiment was conducted to explore the regulation effects of earthworm activity on the soil fungal community under continuous cropping of Sanqi.Eisenia foetida was added to the soil and cow dung was added at 0%(M0)，1%(M1)，2%(M2)，and 5%(M3)，with untreated soil being the control(CK).After 2.5 months of pot experiment，soil samples were collected to determine soil aggregates，physicochemical properties，and enzyme activities.Moreover，high-throughput sequencing technology was used to analyze the composition and diversity of soil fungal communities，and the correlations between soil physicochemical factors and changes in fungal communities were explored.The results showed that M1-M3 treatments increased the content of water-stable macroaggregates.In addition，compared to CK，soil electrical conductivity，organic matter，and available nitrogen，phosphorus，and potassium contents decreased under the M0 treatment；with the increase in the amount of cow manure addition(M1-M3)，the soil electrical conductivity，organic matter，and available nutrient contents increased correspondingly；under M3 treatment，the activities of sucrase，catalase and phosphatase in soil were the highest，which increased by 15.19 mg·g-1，5.55 mg·L-1 and 0.73 mg·g-1，respectively(P<0.05).Compared with CK，the addition of earthworms(M0-M3)reduced the relative abundance of Fusarium，and Plectosphaerella，which were the potential pathogens in soil，by 1.09%-2.27% and 0.21%-0.28%，respectively；however，the relative abundance of potential beneficial fungi Galactomyces and Aspergillus increased by 7.75%-17.70% and 0.15%-0.32%，respectively.Network analysis indicated that earthworm activity increased the complexity of soil fungal communities.Therefore，earthworm activity was expected to be an effective measure to improve the soil health under continuous cropping of Sanqi and reduce the continuous cropping obstacle.