Cyclopentanone(CCO)，as a novel bioinhibitor，boasts multifunctionality and enhanced environmental friendliness，making it more conducive to the green development requirements of modern agriculture compared to traditional chemically synthesized inhibitors. To guide the practical application of CCO，this study employed field experiments，taking Liaoning’s aquic brown soil maize fields as the subject，and quantified greenhouse gas emissions，soil nitrogen regulation capability，plant growth indicators，and yield enhancement effects.By delving into the mechanism and efficacy of CCO，the optimal dosage for its real-world application was scientifically assessed.Five treatments were designed:1)sole urea application(Urea)；2)CCO applied at 0.5% of the nitrogen application rate(CCO1)；3)CCO applied at 1% of the nitrogen application rate(CCO₂)；4)CCO applied at 2% of the nitrogen application rate(CCO3)；5)CCO applied at 5% of the nitrogen application rate(CCO4).Findings revealed that the CCO₂ treatment excelled in controlling greenhouse gas emissions.Relative to the Urea treatment，it achieved a 32.5% reduction in NH₃ emissions and a 21.9% decrease in N2O emissions.Additionally，cumulative CH4 uptake by the soil increased 5.59 times under this treatment.Considering global warming potential and greenhouse gas intensity，the CCO₂ dosage had a significantly greater impact on mitigating the greenhouse effect(P<0.05).Statistical analyses of plant traits，crop yields，and nitrogen use efficiency metrics indicated that CCO₂ more effectively balanced soil nitrogen，optimized crop nitrogen utilization，facilitated plant growth and development，leading to approximately a 20% increase in yield and improved economic returns.CCO，as a new inhibitor，demonstrated remarkable and diverse efficacy，harboring substantial potential for future development.Balancing environmental benefits and economic efficiency，the recommended dosage for CCO application in agricultural production was 1% of the nitrogen applied，based on the comprehensive assessment.