To study the effects of different types of salt-reducing bio-organic fertilizers on the remediation of soil secondary salinization and the growth of Eustoma grandiflorum，two types of bio-organic fertilizers，A type and B type，were made with application amounts of 9，12，15，and 18 t·hm-2，respectively，and conventional fertilization was used as the control to compare the effects of different treatments on the soil physicochemical characteristics，as well as the growth and wilt mortality rate of Eustoma grandiflorum. The results showed that the application of two types of bio-organic fertilizers significantly reduced soil pH，electrical conductivity，and salt content(P<0.05)，and significantly increased soil organic matter content(P<0.05). In addition，with the increase of bio-organic fertilizer application，the plant height，number of flower branches，and quality grade of Eustoma grandiflorum were increased，and the best effect was achieved under the treatment of 15 t·hm-2 application. The mortality rates before the first and second harvest of Eustoma grandiflorum treated with bio-organic fertilizer was significantly decreased(P<0.05). Variance partitioning analysis showed that the application of both A and B type bio-organic fertilizers promoted the growth of Eustoma grandiflorum by regulating the content of available nutrients in soil and reducing soil salinity，with explanatory rates of 74.8% and 59.1%，respectively. Therefore，both A and B type bio-organic fertilizers could effectively reduce the mortality rate of wilt disease，promote the growth of Eustoma grandiflorum，and improve the quality of flower branches；the effect of the second cultivation was more significant.